Evidence map›Paper›PMID 28243520›Full record

ArticleIntravital2016

Intravital multiphoton imaging of mouse tibialis anterior muscle.

Jasmine Lau, Chi Ching Goh, Sapna Devi, Jo Keeble, Peter See, Florent Ginhoux, Lai Guan Ng

Open access · bronzeAbstract read
In one paragraph

Article in Intravital, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.2field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Jasmine LauSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (ASTAR) , Biopolis, Singapore.
Chi Ching GohSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (ASTAR) , Biopolis, Singapore.
Sapna DeviDepartment of Microbiology and Immunology, The Peter Doherty Institute, The University of Melbourne , Parkville, Victoria, Australia.
Jo KeebleDepartment of Medicine, University of Melbourne, Department of Rheumatology, Royal Melbourne Hospital, Inflammation Division, Walter and Eliza Hall Institute of Medical Research , Melbourne, Australia.
Peter SeeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (ASTAR) , Biopolis, Singapore.
Florent GinhouxSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (ASTAR) , Biopolis, Singapore.
Lai Guan NgSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (ASTAR) , Biopolis, Singapore.
Singapore Immunology Network · SGPeter Doherty Institute · AUWalter and Eliza Hall Institute of Medical Research · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intravital imaging by multiphoton microscopy is a powerful tool to gain invaluable insight into tissue biology and function. Here, we provide a step-by-step tissue preparation protocol for imaging the mouse tibialis anterior skeletal muscle. Additionally, we include steps for jugular vein catheterization that allow for well-controlled intravenous reagent delivery. Preparation of the tibialis anterior muscle is minimally invasive, reducing the chances of inducing damage and inflammation prior to imaging. The tibialis anterior muscle is useful for imaging leukocyte interaction with vascular endothelium, and to understand muscle contraction biology. Importantly, this model can be easily adapted to study neuromuscular diseases and myopathies.

Indexed as

fluorescentinflammationmultiphoton imagingskeletal musclevascular

Identifiers

PMID28243520
PMCPMC5226003
OpenAlexW2324345413

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.