Evidence map›Paper›PMID 28243510›Full record

ArticleIntravital2014

Imaging windows for long-term intravital imaging: General overview and technical insights.

Maria Alieva, Laila Ritsma, Randy J Giedt, Ralph Weissleder, Jacco van Rheenen

Abstract read
In one paragraph

Article in Intravital, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.

0numbers the graph read from it
0cells of the map it votes in
109citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

109 citing papers in PubMed.

  1. Article
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  9. Article
  10. Targeted SPP1 Inhibition of Tumor-Associated Myeloid Cells Effectively Decreases Tumor Sizes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  11. Review
  12. Article
  13. Article
  14. Imaging Approaches in Cancer Biology.Cold Spring Harbor perspectives in medicine · 2024
    Review
  15. Review
  16. Article
  17. Article
  18. APL bioengineering · 2024
    Article
  19. Article
  20. Article

49 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Maria AlievaCancer Genomics Netherlands; Hubrecht Institute-KNAW and University Medical Centre Utrecht; CT Utrecht, The Netherlands.
Laila RitsmaCenter for Cancer Research and Center for Regenerative Medicine; Massachusetts General Hospital; Richard B. Simches Research Center; Harvard Medical School; Boston, MA USA; Broad Institute of Harvard and Massachusetts Institute for Technology; Cambridge, MA USA.
Randy J GiedtCenter for Systems Biology; Massachusetts General Hospital; Richard B. Simches Research Center; Harvard Medical School; Boston, MA USA.
Ralph WeisslederCenter for Systems Biology; Massachusetts General Hospital; Richard B. Simches Research Center; Harvard Medical School; Boston, MA USA; Department of Systems Biology; Harvard Medical School; Boston, MA USA.
Jacco van RheenenCancer Genomics Netherlands; Hubrecht Institute-KNAW and University Medical Centre Utrecht; CT Utrecht, The Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intravital microscopy is increasingly used to visualize and quantitate dynamic biological processes at the (sub)cellular level in live animals. By visualizing tissues through imaging windows, individual cells (e.g., cancer, host, or stem cells) can be tracked and studied over a time-span of days to months. Several imaging windows have been developed to access tissues including the brain, superficial fascia, mammary glands, liver, kidney, pancreas, and small intestine among others. Here, we review the development of imaging windows and compare the most commonly used long-term imaging windows for cancer biology: the cranial imaging window, the dorsal skin fold chamber, the mammary imaging window, and the abdominal imaging window. Moreover, we provide technical details, considerations, and trouble-shooting tips on the surgical procedures and microscopy setups for each imaging window and explain different strategies to assure imaging of the same area over multiple imaging sessions. This review aims to be a useful resource for establishing the long-term intravital imaging procedure.

Indexed as

abdominal imaging windowcranial imaging windowdorsal skinfold chamberintravital microscopymammary imaging windowsurgery

Identifiers

PMID28243510
PMCPMC5312719

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.