ArticleProceedings of the National Academy of Sciences of the United States of America2017
Specific phospholipid binding to Na,K-ATPase at two distinct sites.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 63 citations in OpenAlex.
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- Dephosphorylation and ion binding in prokaryotic calcium transport.Science advances · 2024Article
- Monomeric α-synuclein activates the plasma membrane calcium pump.The EMBO journal · 2023Article
- Electrostatic switch mechanisms of membrane protein trafficking and regulation.Biophysical reviews · 2023Review
- Anionic Phospholipids Stimulate the Proton Pumping Activity of the Plant Plasma Membrane P-Type HInternational journal of molecular sciences · 2023Article
- Evidence That Binding of Cyclic GMP to the Extracellular Domain of NKA (Sodium-Potassium ATPase) Mediates Natriuresis.Circulation research · 2023Article
- Docking cholesterol to integral membrane proteins with Rosetta.PLoS computational biology · 2023Article
- Structure-Based Function and Regulation of NCX Variants: Updates and Challenges.International journal of molecular sciences · 2022Review
- Electrostatic interactions between single arginine and phospholipids modulate physiological properties of sarcoplasmic reticulum CaScientific reports · 2022Article
- The Donnan-dominated resting state of skeletal muscle fibers contributes to resilience and longevity in dystrophic fibers.The Journal of general physiology · 2022Article
- Association Study Between Polymorphic Loci in Cholesterol Metabolism Pathway and Gallstone in the Tibetan Population.Frontiers in genetics · 2022Article
- Evaluation of Cardiotonic Steroid Modulation of Cellular Cholesterol and Phospholipid.The Journal of membrane biology · 2021Article
- Effect of Detergents on Activity and Magnesium-Dependent Properties of Different Isoforms of NaBulletin of experimental biology and medicine · 2021Article
- Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins.International journal of molecular sciences · 2021Review
- Tracking Membrane Protein Dynamics in Real Time.The Journal of membrane biology · 2021Review
- Exofacial membrane composition and lipid metabolism regulates plasma membrane P4-ATPase substrate specificity.The Journal of biological chemistry · 2020Article
- The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes.Nature protocols · 2020Article
- Overview on solubilization and lipid reconstitution of Na,K-ATPase: enzyme kinetic and biophysical characterization.Biophysical reviews · 2020Review
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane protein function can be affected by the physical state of the lipid bilayer and specific lipid-protein interactions. For Na,K-ATPase, bilayer properties can modulate pump activity, and, as observed in crystal structures, several lipids are bound within the transmembrane domain. Furthermore, Na,K-ATPase activity depends on phosphatidylserine (PS) and cholesterol, which stabilize the protein, and polyunsaturated phosphatidylcholine (PC) or phosphatidylethanolamine (PE), known to stimulate Na,K-ATPase activity. Based on lipid structural specificity and kinetic mechanisms, specific interactions of both PS and PC/PE have been inferred. Nevertheless, specific binding sites have not been identified definitively. We address this question with native mass spectrometry (MS) and site-directed mutagenesis. Native MS shows directly that one molecule each of 18:0/18:1 PS and 18:0/20:4 PC can bind specifically to purified human Na,K-ATPase (α
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