ArticleProceedings of the National Academy of Sciences of the United States of America2017
Voltage-sensitive rhodol with enhanced two-photon brightness.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 58 citations in OpenAlex.
- Electric field-dependent excited-state twisting in donor-acceptor thiazolothiazole dyes establishes a new class of cell membrane voltage sensors.Chemical science · 2026Article
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- Seeing the Spikes: The Future of Targetable Synthetic Voltage Sensors.ACS chemical neuroscience · 2025Review
- Orientation and Membrane Partition Free Energy of PeT-Based Voltage-Sensitive Dyes from Molecular Simulations.The journal of physical chemistry. B · 2024Article
- Sulfone Rhodamines for Voltage Imaging.Chemistry, an Asian journal · 2022Article
- Raptor downregulation rescues neuronal phenotypes in mouse models of Tuberous Sclerosis Complex.Nature communications · 2022Article
- Toward an integrative neurovascular framework for studying brain networks.Neurophotonics · 2022Article
- Chemical Targeting of Rhodol Voltage-Sensitive Dyes to Dopaminergic Neurons.ACS chemical neuroscience · 2022Article
- Discovering Microcircuit Secrets With Multi-Spot Imaging and Electrophysiological Recordings: The Example of Cerebellar Network Dynamics.Frontiers in cellular neuroscience · 2022Review
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- Optical Estimation of Absolute Membrane Potential Using One- and Two-Photon Fluorescence Lifetime Imaging Microscopy.Bioelectricity · 2021Article
- Measuring Absolute Membrane Potential Across Space and Time.Annual review of biophysics · 2021Review
- Fluorescence lifetime predicts performance of voltage sensitive fluorophores in cardiomyocytes and neurons.RSC chemical biology · 2021Article
- Activity-Based Sensing: A Synthetic Methods Approach for Selective Molecular Imaging and Beyond.Angewandte Chemie (International ed. in English) · 2020Review
- Chemical Targeting of Voltage Sensitive Dyes to Specific Cells and Molecules in the Brain.Journal of the American Chemical Society · 2020Article
- Electrophysiology, Unplugged: Imaging Membrane Potential with Fluorescent Indicators.Accounts of chemical research · 2020Review
- Covalently Tethered Rhodamine Voltage Reporters for High Speed Functional Imaging in Brain Tissue.Journal of the American Chemical Society · 2020Article
- All-Optical Detection of Neuronal Membrane Depolarization in Live Cells Using Colloidal Quantum Dots.Nano letters · 2019Article
- Bioelectrical controls of morphogenesis: from ancient mechanisms of cell coordination to biomedical opportunities.Current opinion in genetics & development · 2019Review
- Guest Editorial.Bioelectricity · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We have designed, synthesized, and applied a rhodol-based chromophore to a molecular wire-based platform for voltage sensing to achieve fast, sensitive, and bright voltage sensing using two-photon (2P) illumination. Rhodol VoltageFluor-5 (RVF5) is a voltage-sensitive dye with improved 2P cross-section for use in thick tissue or brain samples. RVF5 features a dichlororhodol core with pyrrolidyl substitution at the nitrogen center. In mammalian cells under one-photon (1P) illumination, RVF5 demonstrates high voltage sensitivity (28% ΔF/F per 100 mV) and improved photostability relative to first-generation voltage sensors. This photostability enables multisite optical recordings from neurons lacking tuberous sclerosis complex 1, Tsc1, in a mouse model of genetic epilepsy. Using RVF5, we show that Tsc1 KO neurons exhibit increased activity relative to wild-type neurons and additionally show that the proportion of active neurons in the network increases with the loss of Tsc1. The high photostability and voltage sensitivity of RVF5 is recapitulated under 2P illumination. Finally, the ability to chemically tune the 2P absorption profile through the use of rhodol scaffolds affords the unique opportunity to image neuronal voltage changes in acutely prepared mouse brain slices using 2P illumination. Stimulation of the mouse hippocampus evoked spiking activity that was readily discerned with bath-applied RVF5, demonstrating the utility of RVF5 and molecular wire-based voltage sensors with 2P-optimized fluorophores for imaging voltage in intact brain tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.