ArticleBMC biotechnology2017
Enhancing the thermostability of α-L-rhamnosidase from Aspergillus terreus and the enzymatic conversion of rutin to isoquercitrin by adding sorbitol.
Article in BMC biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 48 citations in OpenAlex.
- Methods for the Thermal Stabilization of α-L-Rhamnosidase and Inactivation of β-Glucosidase in the Naringinase Complex fromMolecules (Basel, Switzerland) · 2026Article
- Enhanced Antioxidant and Anti-Inflammatory Activities ofAntioxidants (Basel, Switzerland) · 2025Article
- Heterologous Expression and Characterization of a Thermostable α-L-Rhamnosidase fromJournal of microbiology and biotechnology · 2023Article
- Efficient production of icariin and baohuoside I from Epimedium Folium flavonoids by fungal α-L-rhamnosidase hydrolysing regioselectively the terminal rhamnose of epimedin C.Biotechnology for biofuels and bioproducts · 2023Article
- α-L-rhamnosidase: production, properties, and applications.World journal of microbiology & biotechnology · 2023Review
- Highly Efficient Biotransformation of Notoginsenoside R1 into Ginsenoside Rg1 byJournal of microbiology and biotechnology · 2022Article
- Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules.International journal of molecular sciences · 2019Article
- Nano-encapsulation of naringinase produced by Trichoderma longibrachiatum ATCC18648 on thermally stable biopolymers for citrus juice debittering.Journal of microbiology (Seoul, Korea) · 2019Article
- Effects of β-glucosidase and α-rhamnosidase on the Contents of Flavonoids, Ginkgolides, and Aroma Components in Ginkgo Tea Drink.Molecules (Basel, Switzerland) · 2019Article
- Characterization of a novel thermostable and xylose-tolerant GH 39 β-xylosidase from Dictyoglomus thermophilum.BMC biotechnology · 2018Article
- Enrichment and Purification of Total Ginkgo FlavonoidMolecules (Basel, Switzerland) · 2018Article
- Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability of α-L-rhamnosidase.
resultsIn this study, addition of sorbitol has been found to increase the thermostability of α-L-rhamnosidase from Aspergillus terreus at temperatures ranging from 65 °C to 80 °C. Half-life and activation free energy with addition of 2.0 M sorbitol at 70 °C were increased by 17.2-fold, 8.2 kJ/mol, respectively. The analyses of the results of fluorescence spectroscopy and CD have indicated that sorbitol helped to protect the tertiary and secondary structure of α-L-rhamnosidase. Moreover, the isoquercitrin yield increased from 60.01 to 96.43% with the addition of 1.5 M of sorbitol at 70 °C.
conclusionOur findings provide an effective approach for enhancing the thermostability of α-L-rhamnosidase from Aspergillus terreus, which makes it a good candidate for industrial processes of isoquercitrin preparation.
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