ArticleProtein science : a publication of the Protein Society2017
De novo design of signal sequences to localize cargo to the 1,2-propanediol utilization microcompartment.
Article in Protein science : a publication of the Protein Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Broad Stability and pH-Mediated Reversible Disassembly of In Vitro Assembled Bacterial Microcompartment Shells.Nano letters · 2026Article
- Review
- A blueprint for biomolecular condensation driven by bacterial microcompartment encapsulation peptides.Nature communications · 2025Article
- Article
- Chaotrope-Based Approach for Rapid In Vitro Assembly and Loading of Bacterial Microcompartment Shells.ACS nano · 2025Article
- Vertex protein PduN tunes encapsulated pathway performance by dictating bacterial metabolosome morphology.Nature communications · 2022Article
- Biogenesis of a bacterial metabolosome for propanediol utilization.Nature communications · 2022Article
- Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Encapsulation mechanisms and structural studies of GRM2 bacterial microcompartment particles.Nature communications · 2020Article
- Bioengineered Polyhydroxyalkanoates as Immobilized Enzyme Scaffolds for Industrial Applications.Frontiers in bioengineering and biotechnology · 2020Review
- Engineering the PduT shell protein to modify the permeability of the 1,2-propanediol microcompartment ofMicrobiology (Reading, England) · 2019Article
- Bacterial microcompartments: catalysis-enhancing metabolic modules for next generation metabolic and biomedical engineering.BMC biology · 2019Review
- Nanoreactor Design Based on Self-Assembling Protein Nanocages.International journal of molecular sciences · 2019Review
- Cargo encapsulation in bacterial microcompartments: Methods and analysis.Methods in enzymology · 2019Article
- In Vitro Assembly of Diverse Bacterial Microcompartment Shell Architectures.Nano letters · 2018Article
- Deciphering molecular details in the assembly of alpha-type carboxysome.Scientific reports · 2018Article
- De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments.Nature communications · 2018Article
- Programmed loading and rapid purification of engineered bacterial microcompartment shells.Nature communications · 2018Article
- Engineering nanoreactors using bacterial microcompartment architectures.Current opinion in biotechnology · 2018Review
- Bacterial microcompartments.Nature reviews. Microbiology · 2018Review
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Organizing heterologous biosyntheses inside bacterial cells can alleviate common problems owing to toxicity, poor kinetic performance, and cofactor imbalances. A subcellular organelle known as a bacterial microcompartment, such as the 1,2-propanediol utilization microcompartment of Salmonella, is a promising chassis for this strategy. Here we demonstrate de novo design of the N-terminal signal sequences used to direct cargo to these microcompartment organelles. We expand the native repertoire of signal sequences using rational and library-based approaches and show that a canonical leucine-zipper motif can function as a signal sequence for microcompartment localization. Our strategy can be applied to generate new signal sequences localizing arbitrary cargo proteins to the 1,2-propanediol utilization microcompartments.
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