ArticlePloS one2017
Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Interleukin-6 and leukemia inhibitory factor differentially modify embryonic disk composition in post hatching bovine embryos†.Biology of reproduction · 2026Article
- Non-steroidal anti-inflammatory drugs improve bovine blastocyst formation and quality in in vitro culture.Archives animal breeding · 2025Article
- Interleukin-6 supplementation improves bovine conceptus elongation and transcriptomic indicators of developmental competence†.Biology of reproduction · 2024Article
- Efficient derivation of embryonic stem cells and primordial germ cell-like cells in cattle.The Journal of reproduction and development · 2024Article
- Silencing XIST on the future active X: Searching human and bovine preimplantation embryos for the repressor.European journal of human genetics : EJHG · 2024Article
- Effect of putrescine supplementation to in vitro maturation medium on embryo development and quality in cattle.Animal biotechnology · 2023Article
- Pre-Implantation Bovine Embryo Evaluation-From Optics to Omics and Beyond.Animals : an open access journal from MDPI · 2023Review
- Developmental Hurdles That Can Compromise Pregnancy during the First Month of Gestation in Cattle.Animals : an open access journal from MDPI · 2023Review
- The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization†.Biology of reproduction · 2022Article
- In vitro culture of ovine embryos up to early gastrulating stages.Development (Cambridge, England) · 2022Article
- Reverse transcription priming methods affect normalisation choices for gene expression levels in oocytes and early embryos.Molecular human reproduction · 2021Article
- Article
- Cellular Fragments in the Perivitelline Space Are Not a Predictor of Expanded Blastocyst Quality.Frontiers in cell and developmental biology · 2020Article
- LncRNA-BLACAT1 Facilitates Proliferation, Migration and Aerobic Glycolysis of Pancreatic Cancer Cells by Repressing CDKN1C via EZH2-Induced H3K27me3.Frontiers in oncology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammalian preimplantation development, pluripotent cells are set aside from cells that contribute to extra-embryonic tissues. Although the pluripotent cell population of mouse and human embryos can be cultured as embryonic stem cells, little is known about the pathways involved in formation of a bovine pluripotent cell population, nor how to maintain these cells in vitro. The objective of this study was to determine the transcriptomic profile related to bovine pluripotency. Therefore, in vitro derived embryos were cultured in various culture media that recently have been reported capable of maintaining the naïve pluripotent state of human embryonic cells. Gene expression profiles of embryos cultured in these media were compared using microarray analysis and quantitative RT-PCR. Compared to standard culture conditions, embryo culture in 'naïve' media reduced mRNA expression levels of the key pluripotency markers NANOG and POU5F1. A relatively high percentage of genes with differential expression levels were located on the X-chromosome. In addition, reduced XIST expression was detected in embryos cultured in naïve media and female embryos contained fewer cells with H3K27me3 foci, indicating a delay in X-chromosome inactivation. Whole embryos cultured in one of the media, 5iLA, could be maintained until 23 days post fertilization. Together these data indicate that 'naïve' conditions do not lead to altered expression of known genes involved in pluripotency. Interestingly, X-chromosome inactivation and development of bovine embryos were dependent on the culture conditions.
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