ArticleScientific reports2017
Specialized activities and expression differences for Clostridium thermocellum biofilm and planktonic cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Visual exoproteomics ofbioRxiv : the preprint server for biology · 2026Article
- Differential Proteomic Analysis of Extracellular Vesicles Produced byDentistry journal · 2025Article
- Biochemical and functional properties of vesicles from planktonic and biofilm phenotypes of Limosilactobacillus reuteri DSM 17938.Scientific reports · 2025Article
- Understanding the Impact of Salt Stress on Plant Pathogens Through Phenotypic and Transcriptomic Analysis.Plants (Basel, Switzerland) · 2025Article
- Carbon Source and Substrate Surface Affect Biofilm Formation by the Plant-Associated BacteriumInternational journal of molecular sciences · 2024Article
- The exceptional form and function of the giant bacteriumProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Expression profiling of Clostridium thermocellum B8 during the deconstruction of sugarcane bagasse and straw.World journal of microbiology & biotechnology · 2023Article
- Article
- Sorption of Cellulases in Biofilm Enhances Cellulose Degradation byMicroorganisms · 2022Article
- Utilization of Monosaccharides byMicroorganisms · 2021Article
- Review
- Transcriptomic analysis of a Clostridium thermocellum strain engineered to utilize xylose: responses to xylose versus cellobiose feeding.Scientific reports · 2020Article
- Gut Microbiome Critically Impacts PCB-induced Changes in Metabolic Fingerprints and the Hepatic Transcriptome in Mice.Toxicological sciences : an official journal of the Society of Toxicology · 2020Article
- Impacts of biofilms on the conversion of cellulose.Applied microbiology and biotechnology · 2020Review
- Role ofFrontiers in cellular and infection microbiology · 2020Review
- Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum.Biotechnology letters · 2018Article
- A global analysis of gene expression inBiotechnology for biofuels · 2018Article
- Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium (Ruminiclostridium) thermocellum is a model organism for its ability to deconstruct plant biomass and convert the cellulose into ethanol. The bacterium forms biofilms adherent to lignocellulosic feedstocks in a continuous cell-monolayer in order to efficiently break down and uptake cellulose hydrolysates. We developed a novel bioreactor design to generate separate sessile and planktonic cell populations for omics studies. Sessile cells had significantly greater expression of genes involved in catabolism of carbohydrates by glycolysis and pyruvate fermentation, ATP generation by proton gradient, the anabolism of proteins and lipids and cellular functions critical for cell division consistent with substrate replete conditions. Planktonic cells had notably higher gene expression for flagellar motility and chemotaxis, cellulosomal cellulases and anchoring scaffoldins, and a range of stress induced homeostasis mechanisms such as oxidative stress protection by antioxidants and flavoprotein co-factors, methionine repair, Fe-S cluster assembly and repair in redox proteins, cell growth control through tRNA thiolation, recovery of damaged DNA by nucleotide excision repair and removal of terminal proteins by proteases. This study demonstrates that microbial attachment to cellulose substrate produces widespread gene expression changes for critical functions of this organism and provides physiological insights for two cells populations relevant for engineering of industrially-ready phenotypes.
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