ArticleBiotechnology for biofuels2017
Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast.
Article in Biotechnology for biofuels, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 67 citations in OpenAlex.
- Integrating Machine Learning and Transcriptomics to Enhance β‑Carotene Production inACS omega · 2026Article
- Enhanced production of sabinene by engineered Saccharomyces cerevisiae from corn hydrolysates.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Cyanamide-inducible expression of homing nucleaseSynthetic and systems biotechnology · 2024Article
- Product Profiles of Promiscuous Enzymes Can be Altered by Controlling In Vivo Spatial Organization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Profiling proteomic responses to hexokinase-II depletion in terpene-producingEngineering microbiology · 2023Article
- Article
- Engineering a universal and efficient platform for terpenoid synthesis in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- An outlook to sophisticated technologies and novel developments for metabolic regulation in theFrontiers in bioengineering and biotechnology · 2023Review
- An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae.Nature communications · 2022Article
- Characterizing and engineering promoters for metabolic engineering ofSynthetic and systems biotechnology · 2022Article
- Engineering eukaryote-like regulatory circuits to expand artificial control mechanisms for metabolic engineering in Saccharomyces cerevisiae.Communications biology · 2022Article
- Saccharomyces cerevisiae as a Heterologous Host for Natural Products.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Microbial Production, Extraction, and Quantitative Analysis of Isoprenoids.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Auxin-mediated induction of GAL promoters by conditional degradation of Mig1p improves sesquiterpene production in Saccharomyces cerevisiae with engineered acetyl-CoA synthesis.Microbial biotechnology · 2021Article
- Engineering Posttranslational Regulation of Glutamine Synthetase for Controllable Ammonia Production in the Plant Symbiont Azospirillum brasilense.Applied and environmental microbiology · 2021Article
- Fermentation Strategies for Production of Pharmaceutical Terpenoids in Engineered Yeast.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Auxin-mediated protein depletion for metabolic engineering in terpene-producing yeast.Nature communications · 2021Article
- Compartmentalized Reconstitution of Post-Frontiers in microbiology · 2021Article
- The Model SystemLife (Basel, Switzerland) · 2020Review
- Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAssembly of heterologous metabolic pathways is commonly required to generate microbial cell factories for industrial production of both commodity chemicals (including biofuels) and high-value chemicals. Promoter-mediated transcriptional regulation coordinates the expression of the individual components of these heterologous pathways. Expression patterns vary during culture as conditions change, and this can influence yeast physiology and productivity in both positive and negative ways. Well-characterized strategies are required for matching transcriptional regulation with desired output across changing culture conditions.
resultsHere, constitutive and inducible regulatory mechanisms were examined to optimize synthetic isoprenoid metabolic pathway modules for production of
conclusionDirect comparison of different transcriptional regulatory mechanisms clearly demonstrated that coupling the output strength to the fermentation stage is important to optimize the growth fitness and overall productivities of engineered cells in industrially relevant processes. Applying different well-characterized promoters with the same induction behaviour mitigates against the risks of homologous sequence-mediated genetic instability. Using these approaches, we significantly improved sesquiterpene production in yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.