ArticleACS infectious diseases2017
Discovery of a Novel Nitric Oxide Binding Protein and Nitric-Oxide-Responsive Signaling Pathway in Pseudomonas aeruginosa.
Article in ACS infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 78 citations in OpenAlex.
- Functional organization and regulatory logic of theApplied and environmental microbiology · 2026Review
- A bacterial NO-binding sensor domain evolved through acquisition of a cytochrome-derived c-type heme-binding motif.bioRxiv : the preprint server for biology · 2026Article
- Microbial phenazines: biosynthesis, structural diversity, evolution, regulation, and biological significance.Microbiology and molecular biology reviews : MMBR · 2025Review
- The role of nitric oxide synthase in biofilm formation by Staphylococcus aureus.NPJ biofilms and microbiomes · 2025Article
- A nitric oxide-sensing two-component system regulates a range of infection-related phenotypes inmSphere · 2025Article
- Gas-Binding Studies of Class 1 Sugar Beet Phytoglobin and C86A Mutant Using Isothermal Spectral Shifts in High-Precision Microliter Assay.International journal of molecular sciences · 2025Article
- Tyrosol blocks E. coli anaerobic biofilm formation via YbfA and FNR to increase antibiotic susceptibility.Nature communications · 2024Article
- A c-di-GMP signaling module controls responses to iron in Pseudomonas aeruginosa.Nature communications · 2024Article
- The histidine kinase NahK regulates pyocyanin production through the PQS system.Journal of bacteriology · 2024Article
- Coevolution between marine Aeromonas and phages reveals temporal trade-off patterns of phage resistance and host population fitness.The ISME journal · 2023Article
- Negative regulation of biofilm formation by nitric oxide sensing proteins.Biochemical Society transactions · 2023Review
- NosP Detection of Heme ModulatesBiochemistry · 2023Article
- Gas and light: triggers of c-di-GMP-mediated regulation.FEMS microbiology reviews · 2023Review
- Production of copropophyrin III, biliverdin and bilirubin by the rufomycin producer,Frontiers in microbiology · 2023Article
- Nitric Oxide, Nitric Oxide Formers and Their Physiological Impacts in Bacteria.International journal of molecular sciences · 2022Review
- Nitric oxide stimulates type IV MSHA pilus retraction inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Treatment ofFrontiers in microbiology · 2022Review
- Nitric oxide-secreting probiotics as sustainable bio-cleaners for reverse osmosis membrane systems.Environmental science and pollution research international · 2022Review
- Combination Therapies for Biofilm Inhibition and Eradication: A Comparative Review of Laboratory and Preclinical Studies.Frontiers in cellular and infection microbiology · 2022Review
- Nitric-oxide-driven oxygen release in anoxiciScience · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Nitric oxide (NO) is a radical diatomic gas molecule that, at low concentrations, plays important signaling roles in both eukaryotes and bacteria. In recent years, it has become evident that bacteria respond to low levels of NO in order to modulate their group behavior. Many bacteria respond via NO ligation to a well-established NO sensor called H-NOX (heme-nitric oxide/oxygen binding domain). Many others, such as Pseudomonas aeruginosa, lack an annotated hnoX gene in their genome yet are able to respond to low levels of NO to disperse their biofilms. This suggests the existence of a previously uncharacterized NO sensor. In this study, we describe the discovery of a novel nitric oxide binding protein (NosP; NO-sensing protein), which is much more widely conserved in bacteria than H-NOX, as well as a novel NO-responsive pathway in P. aeruginosa. We demonstrate that biofilms of a P. aeruginosa mutant lacking components of the NosP pathway lose the ability to disperse in response to NO. Upon cloning, expressing, and purifying NosP, we find it binds heme and ligates to NO with a dissociation rate constant that is comparable to that of other well-established NO-sensing proteins. Moreover, we show that NO-bound NosP is able to regulate the phosphorelay activity of a hybrid histidine kinase that is involved in biofilm regulation in P. aeruginosa. Thus, here, we present evidence of a novel NO-responsive pathway that regulates biofilm in P. aeruginosa.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.