Evidence map›Paper›PMID 28235976›Full record

ArticleMedical science monitor : international medical journal of experimental and clinical research2017

Immune Regulation of Intrahepatic Regulatory T Cells in Fibrotic Livers of Mice.

Xiaohui Zhang, Jinli Lou, Li Bai, Yu Chen, Sujun Zheng, Zhongping Duan

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical science monitor : international medical journal of experimental and clinical research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 23 citations in OpenAlex.

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  16. Mesenchymal stem cell therapy for liver fibrosis/cirrhosis.Annals of translational medicine · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Xiaohui ZhangArtificial Liver Treatment Center, Beijing YouAn Hospital, Capital Medical University, Beijing, China (mainland).
Jinli LouClinical Laboratory Center, Beijing YouAn Hospital, Capital Medical University, Beijing, China (mainland).
Li BaiArtificial Liver Treatment Center, Beijing YouAn Hospital, Capital Medical University, Beijing, China (mainland).
Yu ChenArtificial Liver Treatment Center, Beijing YouAn Hospital, Capital Medical University, Beijing, China (mainland).
Sujun ZhengArtificial Liver Treatment Center, Beijing YouAn Hospital, Capital Medical University, Beijing, China (mainland).
Zhongping DuanArtificial Liver Treatment Center, Beijing YouAn Hospital, Capital Medical University, Beijing, China (mainland).
Beijing YouAn Hospital · CNCapital Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND Liver fibrosis is the result of chronic inflammation and repair, and many immune cells contribute to the process. Regulatory T cells (Tregs) mediate immune tolerance and are highly expressed in liver fibrosis. However, few reports have studied the specific effects of Tregs on regulating immune cells in liver fibrosis. The present study aimed to investigate the regulation of Tregs on intrahepatic immune cells in liver fibrosis by depleting Tregs in mice. MATERIAL AND METHODS Liver fibrosis was induced by carbon tetrachloride, and an anti-CD25 mAb (PC61) was used to deplete Tregs. Liver fibrosis and injury were reflected by immunofluorescence staining and alanine aminotransferase level. The expressions of immune cell Tregs and cytokines were detected by flow cytometry and/or real-time PCR. Interferon-γ (IFN-γ) concentration was measured by ELISA. RESULTS Tregs were rich in fibrotic livers; after Tregs depletion, the intrahepatic CD4+ T cell and Kupffer cells (KC) populations did not change compared with liver fibrosis, but CD8+ T cells were slightly elevated. However, natural killer (NK) cells and IFN-g levels were significantly decreased in fibrosis and increased after Tregs depletion. Interesting, we found Tregs promoted KC M1/M2 balance to M2, because inducible nitric oxide synthase (M1) was increased but arginase-1 (M2) was reduced after depleting Tregs. Furthermore, in isolated KCs from livers, IL-12 (M1) was increased, but TGF-β (M2) was reduced after depleting Tregs, compared with fibrotic livers. CONCLUSIONS Tregs are involved in the immune regulation of liver fibrosis, primarily by suppressing NK cells and M1 KCs, and mildly suppressing CD8+ T cells.

Indexed as

AnimalsCD4-Positive T-LymphocytesCytokinesDisease Models, AnimalImmune ToleranceInterferon-gammaKiller Cells, NaturalKupffer CellsLiver CirrhosisMaleMiceMice, Inbred C57BLT-Lymphocytes, RegulatoryTransforming Growth Factor betaCytokinesInterferon-gammaTransforming Growth Factor beta

Identifiers

PMID28235976
PMCPMC5338567
OpenAlexW2591243235

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.