Evidence map›Paper›PMID 28235791›Full record

ArticleAmerican journal of physiology. Heart and circulatory physiology2017

High-throughput screening identifies microRNAs that target Nox2 and improve function after acute myocardial infarction.

Junyu Yang, Milton E Brown, Hanshuo Zhang, Mario Martinez, Zhihua Zhao, Srishti Bhutani, Shenyi Yin, David Trac, Jianzhong Jeff Xi, Michael E Davis

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Heart and circulatory physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed, 1 pooled it
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.

  1. Guidelines for experimental models of myocardial ischemia and infarction.American journal of physiology. Heart and circulatory physiology · 2018
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  9. Nanomedicines for cardiovascular disease.Nature cardiovascular research · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Junyu YangDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, China.
Milton E BrownWallace H. Coulter Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, Atlanta, Georgia.
Hanshuo ZhangDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, China.
Mario MartinezWallace H. Coulter Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, Atlanta, Georgia.
Zhihua ZhaoDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, China.
Srishti BhutaniWallace H. Coulter Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, Atlanta, Georgia.
Shenyi YinDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, China.
David TracWallace H. Coulter Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, Atlanta, Georgia.
Jianzhong Jeff XiDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, China.
Michael E DavisDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, China; michael.davis@bme.emory.edu.
Georgia Institute of Technology · USPeking University · CNEmory University · USThe Wallace H. Coulter Department of Biomedical Engineering · US

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM008169 · NIGMS · EMORY UNIVERSITY · PI GROSS, ROBERT E · 1987 to 2021
$20.3M
NIGMS NIH HHS T32 GM008169
6 · The paper itself

Abstract

Myocardial infarction (MI) is the most common cause of heart failure. Excessive production of ROS plays a key role in the pathogenesis of cardiac remodeling after MI. NADPH with NADPH oxidase (Nox)2 as the catalytic subunit is a major source of superoxide production, and expression is significantly increased in the infarcted myocardium, especially by infiltrating macrophages. While microRNAs (miRNAs) are potent regulators of gene expression and play an important role in heart disease, there still lacks efficient ways to identify miRNAs that target important pathological genes for treating MI. Thus, the overall objective was to establish a miRNA screening and delivery system for improving heart function after MI using Nox2 as a critical target. With the use of the miRNA-target screening system composed of a self-assembled cell microarray (SAMcell), three miRNAs, miR-106b, miR-148b, and miR-204, were identified that could regulate Nox2 expression and its downstream products in both human and mouse macrophages. Each of these miRNAs were encapsulated into polyketal (PK3) nanoparticles that could effectively deliver miRNAs into macrophages. Both in vitro and in vivo studies in mice confirmed that PK3-miRNAs particles could inhibit Nox2 expression and activity and significantly improve infarct size and acute cardiac function after MI. In conclusion, our results show that miR-106b, miR-148b, and miR-204 were able to improve heart function after myocardial infarction in mice by targeting Nox2 and possibly altering inflammatory cytokine production. This screening system and delivery method could have broader implications for miRNA-mediated therapeutics for cardiovascular and other diseases.

Indexed as

AnimalsCell LineGene Expression RegulationGenetic TherapyGene Transfer TechniquesHigh-Throughput Screening AssaysHumansMacrophagesMaleMembrane GlycoproteinsMiceMice, Inbred C57BLMicroRNAsMyocardial InfarctionNADPH Oxidase 2NADPH OxidasesCYBB protein, humanMembrane GlycoproteinsMicroRNAsMirn148 microRNA, mouseMIRN204 microRNA, mouseNADPH Oxidase 2NADPH OxidasesSuperoxidesmicroRNAmyocardial infarctionoxidative stress

Identifiers

PMID28235791
PMCPMC5451584
OpenAlexW2590111829

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.