Evidence map›Paper›PMID 28235582›Full record

ArticleDevelopmental biology2017

Repeated removal of developing limb buds permanently reduces appendage size in the highly-regenerative axolotl.

Donald M Bryant, Konstantinos Sousounis, Johanna E Farkas, Sevara Bryant, Neng Thao, Anna R Guzikowski, James R Monaghan, Michael Levin, Jessica L Whited

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Integrative biology of injury in animals.Biological reviews of the Cambridge Philosophical Society · 2023
    Review
  5. Article
  6. Review
  7. Article
  8. Article
  9. Toward whole tissue imaging of axolotl regeneration.Developmental dynamics : an official publication of the American Association of Anatomists · 2021
    Review
  10. Towards comparative analyses of salamander limb regeneration.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2021
    Review
  11. Review
  12. Review
  13. Article
  14. Review
  15. Model systems for regeneration: salamanders.Development (Cambridge, England) · 2019
    Review
  16. Review
  17. Article
  18. Review
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Donald M BryantHarvard Medical School, the Harvard stem Cell Institute, and the Department of Orthopedic Surgery, Brigham & Women's Hospital, Cambridge, MA 02139, USA.
Konstantinos SousounisHarvard Medical School, the Harvard stem Cell Institute, and the Department of Orthopedic Surgery, Brigham & Women's Hospital, Cambridge, MA 02139, USA; Allen Discovery Center at Tufts, Medford, MA 02155, USA.
Johanna E FarkasDepartment of Biology, Northeastern University, Boston, MA 02115, USA.
Sevara BryantHarvard Medical School, the Harvard stem Cell Institute, and the Department of Orthopedic Surgery, Brigham & Women's Hospital, Cambridge, MA 02139, USA.
Neng ThaoHarvard Medical School, the Harvard stem Cell Institute, and the Department of Orthopedic Surgery, Brigham & Women's Hospital, Cambridge, MA 02139, USA.
Anna R GuzikowskiHarvard Medical School, the Harvard stem Cell Institute, and the Department of Orthopedic Surgery, Brigham & Women's Hospital, Cambridge, MA 02139, USA.
James R MonaghanDepartment of Biology, Northeastern University, Boston, MA 02115, USA.
Michael LevinAllen Discovery Center at Tufts, Medford, MA 02155, USA; Tufts Center for Regenerative and Developmental Biology, Medford, MA 02155, USA.
Jessica L WhitedHarvard Medical School, the Harvard stem Cell Institute, and the Department of Orthopedic Surgery, Brigham & Women's Hospital, Cambridge, MA 02139, USA; Allen Discovery Center at Tufts, Medford, MA 02155, USA. Electronic address: jwhited@bwh.harvard.edu.

Funding

Leveraging Single-Cell Analysis to Elucidate Mechanisms of Vertebrate LimbRegenerationDP2HD087953 · NICHD · HARVARD UNIVERSITY · PI WHITED, JESSICA L. · 2015 to 2020
$3.2M
NICHD NIH HHS DP2 HD087953
6 · The paper itself

Abstract

Matching appendage size to body size is fundamental to animal function. Generating an appropriately-sized appendage is a robust process executed during development which is also critical for regeneration. When challenged, larger animals are programmed to regenerate larger limbs than smaller animals within a single species. Understanding this process has important implications for regenerative medicine. To approach this complex question, models with altered appendage size:body size ratios are required. We hypothesized that repeatedly challenging axolotls to regrow limb buds would affect their developmental program resulting in altered target morphology. We discovered that after 10 months following this experimental procedure, limbs that developed were permanently miniaturized. This altered target morphology was preserved upon amputation and regeneration. Future experiments using this platform should provide critical information about how target limb size is encoded within limb progenitors.

Indexed as

Amputation, SurgicalAmbystoma mexicanumAnimalsEctromeliaLimb BudsNerve TissueOrgan SizeRegenerationAxolotlDevelopmentGrowthLimbMorphogenesisRegeneration

Identifiers

PMID28235582
PMCPMC5707178

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.