ArticlePloS one2017
The best of both worlds: A combined approach for analyzing microalgal diversity via metabarcoding and morphology-based methods.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Method comparison of microscopy, metabarcoding, and multispectral imaging flow cytometry for identification and relative abundance analysis of insect-dispersed pollen.Scientific reports · 2026Article
- The dilemma of underestimating freshwater biodiversity: morphological and molecular approaches.BMC ecology and evolution · 2024Article
- Salinity-induced succession of phototrophic communities in a southwestern Siberian soda lake during the solar activity cycle.Heliyon · 2024Article
- Review
- Structure and Dynamics of Periphyton in a Neotropical Freshwater Lake, with Emphasis on Ciliates and Their Relationships with Bacterial Taxa.Microbial ecology · 2023Article
- Genotyping by sequencing for estimating relative abundances of diatom taxa in mock communities.BMC ecology and evolution · 2023Article
- First insights into the gut microbiomes and the diet of theEvolutionary applications · 2023Article
- Seasonal Diversity of Microeukaryotes in the Han River, Korea Through 18S rRNA Gene Metabarcoding.Evolutionary bioinformatics online · 2022Article
- Altitudinal Zonation of Green Algae Biodiversity in the French Alps.Frontiers in plant science · 2021Article
- Omics for Bioprospecting and Drug Discovery from Bacteria and Microalgae.Antibiotics (Basel, Switzerland) · 2020Review
- µgreen-db: a reference database for the 23S rRNA gene of eukaryotic plastids and cyanobacteria.Scientific reports · 2020Article
- The impact of OTU sequence similarity threshold on diatom-based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean).Ecology and evolution · 2019Article
- Combining morpho-taxonomy and metabarcoding enhances the detection of non-indigenous marine pests in biofouling communities.Scientific reports · 2018Article
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Authors and funding
3 authors.
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Abstract
An increasing number of studies use next generation sequencing (NGS) to analyze complex communities, but is the method sensitive enough when it comes to identification and quantification of species? We compared NGS with morphology-based identification methods in an analysis of microalgal (periphyton) communities. We conducted a mesocosm experiment in which we allowed two benthic grazer species to feed upon benthic biofilms, which resulted in altered periphyton communities. Morphology-based identification and 454 (Roche) pyrosequencing of the V4 region in the small ribosomal unit (18S) rDNA gene were used to investigate the community change caused by grazing. Both the NGS-based data and the morphology-based method detected a marked shift in the biofilm composition, though the two methods varied strongly in their abilities to detect and quantify specific taxa, and neither method was able to detect all species in the biofilms. For quantitative analysis, we therefore recommend using both metabarcoding and microscopic identification when assessing the community composition of eukaryotic microorganisms.
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