ArticlePloS one2017
Does metabolite channeling accelerate enzyme-catalyzed cascade reactions?
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Liquid-Liquid Phase Separation-Enhanced Multienzyme Catalysis: Mechanisms and Applications.ChemSusChem · 2026Review
- Complete enzyme clustering enhances coenzyme Q biosynthesis via substrate channeling.Nature communications · 2026Article
- Exploring linker's sequence diversity to fuse carotene cyclase and hydroxylase for zeaxanthin biosynthesis.Metabolic engineering communications · 2023Article
- From Protein Film Electrochemistry to Nanoconfined Enzyme Cascades and the Electrochemical Leaf.Chemical reviews · 2023Review
- Riboflavin kinase and pyridoxine 5'-phosphate oxidase complex formation envisages transient interactions for FMN cofactor delivery.Frontiers in molecular biosciences · 2023Article
- Mechanisms and Effects of Substrate Channelling in Enzymatic Cascades.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Genetic fusion of P450 BM3 and formate dehydrogenase towards self-sufficient biocatalysts with enhanced activity.Scientific reports · 2021Article
- Metabolic channeling: predictions, deductions, and evidence.Molecular cell · 2021Review
- Cluster Channeling in Cascade Reactions.The journal of physical chemistry. B · 2021Article
- Review
- Refining Metabolic Mass Transfer for Efficient Biosynthesis of Plant Natural Products in Yeast.Frontiers in bioengineering and biotechnology · 2021Review
- A novel terpene synthase controls differences in anti-aphrodisiac pheromone production between closely related Heliconius butterflies.PLoS biology · 2021Article
- Substrate Channeling via a Transient Protein-Protein Complex: The case of D-Glyceraldehyde-3-Phosphate Dehydrogenase and L-Lactate Dehydrogenase.Scientific reports · 2020Article
- Construction of a fusion enzyme for astaxanthin formation and its characterisation in microbial and plant hosts: A new tool for engineering ketocarotenoids.Metabolic engineering · 2019Article
- Can enzyme proximity accelerate cascade reactions?Scientific reports · 2019Article
- Enzymatic complexes across scales.Essays in biochemistry · 2018Review
- The role of dynamic enzyme assemblies and substrate channelling in metabolic regulation.Nature communications · 2018Review
- Engineered synthetic scaffolds for organizing proteins within the bacterial cytoplasm.Nature chemical biology · 2018Article
- Spatial Organization of Metabolic Enzyme Complexes in Cells.Biochemistry · 2017Review
- Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolite or substrate channeling is a direct transfer of metabolites from one enzyme to the next enzyme in a cascade. Among many potential advantages of substrate channeling, acceleration of the total reaction rate is considered as one of the most important and self-evident. However, using a simple model, supported by stochastic simulations, we show that it is not always the case; particularly at long times (i.e. in steady state) and high substrate concentrations, a channeled reaction cannot be faster, and can even be slower, than the original non-channeled cascade reaction. In addition we show that increasing the degree of channeling may lead to an increase of the metabolite pool size. We substantiate that the main advantage of channeling likely lies in protecting metabolites from degradation or competing side reactions.
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