ArticlePloS one2017
Can milk cell or skim milk miRNAs be used as biomarkers for early pregnancy detection in cattle?
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- Investigating Dietary microRNA Stability and Function Using a Transgenic Milk Model With Unique microRNA Sequences.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Heat stress modulates extracellular vesicles miRNA cargo in bovine uterine fluid and uterine function.Frontiers in veterinary science · 2026Article
- First insights into the miRNA landscape of Tursiops truncatus milk reveal shared dominant microRNA families with terrestrial mammals.PloS one · 2026Article
- Differential Expression of miR-223-3p and miR-26-5p According to Different Stages of Mastitis in Dairy Cows.Biomolecules · 2025Article
- Analysis of miRNAs in milk of four livestock species.BMC genomics · 2024Article
- Dairy Products: Processing Technology and Sensory Properties.Foods (Basel, Switzerland) · 2024Article
- Identification of Hub Genes and Target miRNAs Crucial for Milk Production in Holstein Friesian Dairy Cattle.Genes · 2023Article
- Association between blood miR-26a levels following artificial insemination, and pregnancy outcome in dairy cattle.PloS one · 2023Article
- MiRNAs in milk can be used towards early prediction of mammary gland inflammation in cattle.Scientific reports · 2022Article
- Determining extracellular vesicles properties and miRNA cargo variability in bovine milk from healthy cows and cows undergoing subclinical mastitis.BMC genomics · 2022Article
- MicroRNAs in amniotic fluid and maternal blood plasma associated with sex determination and early gonad differentiation in cattle†.Biology of reproduction · 2021Article
- Feasibility and accuracy of using different methods to detect pregnancy by conceptus-stimulated genes in dairy cattle.JDS communications · 2021Article
- The Importance of Interferon-Tau in the Diagnosis of Pregnancy.BioMed research international · 2021Review
- Establishment of Repertoire of Placentome-Associated MicroRNAs and Their Appearance in Blood Plasma Could Identify Early Establishment of Pregnancy in Buffalo (Frontiers in cell and developmental biology · 2021Article
- Comparison of a Barium Chloride Test with ELISA for Pregnancy Detection in Cows.Journal of veterinary research · 2020Article
- New approaches to diagnose and target reproductive failure in cattle.Animal reproduction · 2020Review
- Farmer and Veterinary Practices and Opinions Related to Fertility Testing and Pregnancy Diagnosis of UK Dairy Cows.Frontiers in veterinary science · 2020Article
- MicroRNA profiles of dry secretions through the first three weeks of the dry period from Holstein cows.Scientific reports · 2019Article
- Exosomes of pasteurized milk: potential pathogens of Western diseases.Journal of translational medicine · 2019Review
- Genetic and epigenetic architecture of paternal origin contribute to gestation length in cattle.Communications biology · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The most critical phase of pregnancy is the first three weeks following insemination. During this period about 50% of high yielding lactating cows suffer embryonic loss prior to implantation, which poses a high economic burden on dairy farmers. Early diagnosis of pregnancy in cattle is therefore essential for monitoring breeding outcome and efficient production intervals. Regulated microRNAs (miRNAs) that reach easily accessible body fluids via a 'liquid biopsy' could be a new class of pregnancy predicting biomarkers. As milk is obtained regularly twice daily and non-invasively from the animal, it represents an ideal sample material. Our aim was to establish a pregnancy test system based on the discovery of small RNA biomarkers derived from the bovine milk cellular fraction and skim milk of cows. Milk samples were taken on days 4, 12 and 18 of cyclic cows and after artificial insemination, respectively, of the same animals (n = 6). miRNAs were analysed using small RNA sequencing (small RNA Seq). The miRNA profiles of milk cells and skim milk displayed similar profiles despite the presence of immune cell related miRNAs in milk cells. Trends in regulation of miRNAs between the oestrous cycle and pregnancy were found in miR-cluster 25~106b and its paralog cluster 17~92, miR-125 family, miR-200 family, miR-29 family, miR-15a, miR-21, miR-26b, miR-100, miR-140, 193a-5p, miR-221, miR-223, miR-320a, miR-652, miR-2898 and let-7i. A separation of cyclic and pregnant animals was achieved in a principal component analysis. Bta-miRs-29b, -221, -125b and -200b were successfully technically validated using quantitative real-time PCR, however biological validation failed. Therefore we cannot recommend the diagnostic use of these miRNAs in milk as biomarkers for detection of bovine pregnancy for now.
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