Evidence map›Paper›PMID 28234924›Full record

ArticlePloS one2017

DNApod: DNA polymorphism annotation database from next-generation sequence read archives.

Takako Mochizuki, Yasuhiro Tanizawa, Takatomo Fujisawa, Tazro Ohta, Naruo Nikoh, Tokurou Shimizu, Atsushi Toyoda, Asao Fujiyama, Nori Kurata, Hideki Nagasaki and 2 more

Abstract read
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Takako MochizukiGenome Informatics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
Yasuhiro TanizawaGenome Informatics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
Takatomo FujisawaGenome Informatics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
Tazro OhtaDatabase Center for Life Science, Joint Support-Center for Data Science Research, Research Organization of Information and Systems, Mishima, Shizuoka, Japan.
Naruo NikohDepartment of Liberal Arts, The Open University of Japan, Chiba, Chiba, Japan.
Tokurou ShimizuDivision of Citrus Research, Institute of Fruit Tree and Tea Science, NARO, Shimizu, Shizuoka, Japan.
Atsushi ToyodaComparative Genomics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
Asao FujiyamaAdvanced Genomics Center, National Institute of Genetics, Mishima, Shizuoka, Japan.
Nori KurataPlant Genetics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
Hideki NagasakiGenome Informatics Group, Department of Technology Development, Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
Eli KaminumaGenome Informatics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.
Yasukazu NakamuraGenome Informatics Laboratory, National Institute of Genetics, Mishima, Shizuoka, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

With the rapid advances in next-generation sequencing (NGS), datasets for DNA polymorphisms among various species and strains have been produced, stored, and distributed. However, reliability varies among these datasets because the experimental and analytical conditions used differ among assays. Furthermore, such datasets have been frequently distributed from the websites of individual sequencing projects. It is desirable to integrate DNA polymorphism data into one database featuring uniform quality control that is distributed from a single platform at a single place. DNA polymorphism annotation database (DNApod; http://tga.nig.ac.jp/dnapod/) is an integrated database that stores genome-wide DNA polymorphism datasets acquired under uniform analytical conditions, and this includes uniformity in the quality of the raw data, the reference genome version, and evaluation algorithms. DNApod genotypic data are re-analyzed whole-genome shotgun datasets extracted from sequence read archives, and DNApod distributes genome-wide DNA polymorphism datasets and known-gene annotations for each DNA polymorphism. This new database was developed for storing genome-wide DNA polymorphism datasets of plants, with crops being the first priority. Here, we describe our analyzed data for 679, 404, and 66 strains of rice, maize, and sorghum, respectively. The analytical methods are available as a DNApod workflow in an NGS annotation system of the DNA Data Bank of Japan and a virtual machine image. Furthermore, DNApod provides tables of links of identifiers between DNApod genotypic data and public phenotypic data. To advance the sharing of organism knowledge, DNApod offers basic and ubiquitous functions for multiple alignment and phylogenetic tree construction by using orthologous gene information.

Indexed as

Databases, Nucleic AcidPolymorphism, GeneticCrops, AgriculturalDNADNA, PlantGenes, PlantHigh-Throughput Nucleotide SequencingHomozygoteMolecular Sequence AnnotationOryzaPhenotypePhylogenyReference ValuesReproducibility of ResultsSoftwareSorghumDNADNA, Plant

Identifiers

PMID28234924
PMCPMC5325239

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.