ArticleScientific reports2017
Long-term homeostasis and wound healing in an in vitro epithelial stem cell niche model.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Stem Cell and Exosome Therapy in Wound Healing: Traps, Paradoxes, and Tricks Transforming Paradigms.Biomedicines · 2025Article
- Human corneal organoid has a limbal function that supplies epithelium to the cornea with limbal deficiency.Regenerative therapy · 2025Article
- Identification of a Potential PGK1 Inhibitor with the Suppression of Breast Cancer Cells Using Virtual Screening and Molecular Docking.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Keratin 15 protects against cigarette smoke-induced epithelial mesenchymal transformation by MMP-9.Respiratory research · 2023Article
- Genetic prescreening of a candidate for laser refractive surgery identifies risk for inadequate tissue response: a case report.Journal of medical case reports · 2022Article
- Identification, Isolation, and Characterization of Melanocyte Precursor Cells in the Human Limbal Stroma.International journal of molecular sciences · 2022Article
- Synthesis of Poly(acrylic acid)-Cysteine-Based Hydrogels with Highly Customizable Mechanical Properties for Advanced Cell Culture Applications.ACS omega · 2022Article
- The immunologic changes during different phases of intestinal anastomotic healing.Journal of clinical laboratory analysis · 2020Review
- Isolation and enrichment of melanocytes from human corneal limbus using CD117 (c-Kit) as selection marker.Scientific reports · 2020Article
- Laminin-511-E8 promotes efficient in vitro expansion of human limbal melanocytes.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cultures of epithelial cells are limited by the proliferative capacity of primary cells and cell senescence. Herein we show that primary human epithelial cell sheets cultured without dermal equivalents maintained homeostasis in vitro for at least 1 year. Transparency of these sheets enabled live observation of pigmented melanocytes and Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) labeled epithelial cells during wound healing. Cell turn over and KRT15 expression pattern stabilized within 3 months, when KRT15 bright clusters often associated with niche-like melanocytes became apparent. EdU labels were retained in a subset of epithelial cells and melanocytes after 6 months chasing, suggesting their slow cell cycling property. FUCCI-labeling demonstrated robust cell migration and proliferation following wounding. Transparency and long-term (1 year) homeostasis of this model will be a powerful tool for the study of wound healing and cell linage tracing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.