ArticleAMB Express2017
Loop-mediated isothermal amplification of Neisseria gonorrhoeae porA pseudogene: a rapid and reliable method to detect gonorrhea.
Article in AMB Express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- A low-cost point-of-care device for the simultaneous detection of two sexually transmitted bacterial pathogens in vaginal swab samples.The Analyst · 2025Article
- Clinical Validation of Loop-Mediated Isothermal Amplification Assays for the Rapid Detection of Neisseria gonorrhoeae and Chlamydia trachomatis.Microbial biotechnology · 2025Article
- Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Sexually Transmitted Infections: A Review.Microbial biotechnology · 2025Review
- Rethinking the application of nanoparticles in women's reproductive health and assisted reproduction.Nanomedicine (London, England) · 2024Review
- Promoting molecular diagnostic equity: assessing in-house real-time PCR forLe infezioni in medicina · 2024Article
- Integrated microscale immiscible phase extraction and isothermal amplification for colorimetric detection of Neisseria gonorrhoeae.Analytical and bioanalytical chemistry · 2023Article
- Visual and rapid identification ofFrontiers in cellular and infection microbiology · 2023Article
- Diagnosis ofIndian journal of microbiology · 2022Article
- Comparative analysis of loop-mediated isothermal amplification combined with microfluidic chip technology and q-PCR in the detection of clinical infectious pathogens.Journal of clinical laboratory analysis · 2022Article
- Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of Ureaplasma urealyticum.AMB Express · 2022Article
- Bridging the gap between development of point-of-care nucleic acid testing and patient care for sexually transmitted infections.Lab on a chip · 2022Review
- High-Resolution Melting Analysis to Detect Antimicrobial Resistance Determinants in South AfricanInternational journal of microbiology · 2022Article
- Capillary-based reverse transcriptase loop-mediated isothermal amplification for cost-effective and rapid point-of-care COVID-19 testing.Analytica chimica acta · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectiveGonorrhea is a sexually transmitted disease caused by the bacterium Neisseria gonorrhoeae. Rapid detection is crucial for effective prevention and treatment. This study developed and tested a low-cost effective method for detecting N. gonorrhoeae, especially in developing countries.
methodsDNA from a N. gonorrhoeae standard strain, as well as from 26 genital secretion samples of gonorrhea patients, were isolated and used for loop-mediated isothermal amplification (LAMP) assay, which was conducted using either an automatic real-time PCR analyzer or a water bath. The amplified porA pseudogene sequence was compared with the NCBI database and the LAMP results were compared with that of the traditional culture method for its sensitivity and specificity.
resultsLAMP was able to detect Neisseria DNA at a concentration as low as 1 pg/µL (1 × 10
conclusionLAMP was sensitive and reliable for detecting the porA gene of N. gonorrhoeae. It could be used as a rapid, low cost, and effective method for detecting N. gonorrhoeae.
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