ArticleJournal of molecular evolution2017
Experimental Evolution of Escherichia coli Harboring an Ancient Translation Protein.
Article in Journal of molecular evolution, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Resurrecting tRNAs of the Last Universal Common Ancestor (Commonote) Toward Rebuilding the Ancient Translation System.Journal of molecular evolution · 2026Article
- Macrolide resistance trajectories across threeFrontiers in microbiology · 2026Article
- The origins and evolution of translation factors.Trends in genetics : TIG · 2025Review
- Fitness benefits of a synonymous substitution in an ancient EF-Tu gene depend on the genetic background.Journal of bacteriology · 2024Article
- Experimental evolution for cell biology.Trends in cell biology · 2023Review
- Early divergence of translation initiation and elongation factors.Protein science : a publication of the Protein Society · 2022Article
- Surface display of carbonic anhydrase onSynthetic and systems biotechnology · 2022Article
- An Integrated Method to Reconstruct Ancient Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The cost of evolved constitutiveEcology and evolution · 2021Article
- Contingency and chance erase necessity in the experimental evolution of ancestral proteins.eLife · 2021Article
- Corrigendum: Ancestral Sequence Reconstructions of MotB Are Proton-Motile and Require MotA for Motility.Frontiers in microbiology · 2021Article
- Evolutionary stalling and a limit on the power of natural selection to improve a cellular module.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Evolutionary Repair Experiments as a Window to the Molecular Diversity of Life.Current biology : CB · 2020Review
- Emerging Frontiers in the Study of Molecular Evolution.Journal of molecular evolution · 2020Article
- Ancestral Sequence Reconstructions of MotB Are Proton-Motile and Require MotA for Motility.Frontiers in microbiology · 2020Article
- Heterologous Hsp90 promotes phenotypic diversity through network evolution.PLoS biology · 2018Article
- Experimental Design, Population Dynamics, and Diversity in Microbial Experimental Evolution.Microbiology and molecular biology reviews : MMBR · 2018Review
- Exoplanet Biosignatures: Future Directions.Astrobiology · 2018Review
- Back to the future in a petri dish: Origin and impact of resurrected microbes in natural populations.Evolutionary applications · 2018Review
- Resurrecting ancestral genes in bacteria to interpret ancient biosignatures.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2017Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to design synthetic genes and engineer biological systems at the genome scale opens new means by which to characterize phenotypic states and the responses of biological systems to perturbations. One emerging method involves inserting artificial genes into bacterial genomes and examining how the genome and its new genes adapt to each other. Here we report the development and implementation of a modified approach to this method, in which phylogenetically inferred genes are inserted into a microbial genome, and laboratory evolution is then used to examine the adaptive potential of the resulting hybrid genome. Specifically, we engineered an approximately 700-million-year-old inferred ancestral variant of tufB, an essential gene encoding elongation factor Tu, and inserted it in a modern Escherichia coli genome in place of the native tufB gene. While the ancient homolog was not lethal to the cell, it did cause a twofold decrease in organismal fitness, mainly due to reduced protein dosage. We subsequently evolved replicate hybrid bacterial populations for 2000 generations in the laboratory and examined the adaptive response via fitness assays, whole genome sequencing, proteomics, and biochemical assays. Hybrid lineages exhibit a general adaptive strategy in which the fitness cost of the ancient gene was ameliorated in part by upregulation of protein production. Our results suggest that an ancient-modern recombinant method may pave the way for the synthesis of organisms that exhibit ancient phenotypes, and that laboratory evolution of these organisms may prove useful in elucidating insights into historical adaptive processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.