Evidence map›Paper›PMID 28232590›Full record

ArticleStroke2017

MicroRNA cluster miR-17-92 Cluster in Exosomes Enhance Neuroplasticity and Functional Recovery After Stroke in Rats.

Hongqi Xin, Mark Katakowski, Fengjie Wang, Jian-Yong Qian, Xian Shuang Liu, Meser M Ali, Benjamin Buller, Zheng Gang Zhang, Michael Chopp

Erratum issued Registry-linked trialOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stroke, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. It is linked to trial NCT07398612 (Clinical Study on the Safety and Efficacy of Human Adipose-Derived Stem Cell Exosomes in the Treatment of Acute Ischemic Stroke), which is not on this map. Cited by 339 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
339citing papers in PubMed, 4 pooled it
20.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT07398612 phase1 / phase2not yet recruitingnot on this mapstarted 2026, after this paper: background citation

Clinical Study on the Safety and Efficacy of Human Adipose-Derived Stem Cell Exosomes in the Treatment of Acute Ischemic Stroke

TypeinterventionalSponsorShanghai General Hospital, Shanghai Jiao Tong University School of MedicineRan2026 to 2028Enrolled60ConditionsAcute Ischemic StrokeArmsHuman Adipose-Derived Stem Cell Exosomes, Placebo
3 · Its place in the literature

Who cites it

339 citing papers in PubMed, 4 syntheses or guidelines pooled it, 535 citations in OpenAlex.

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279 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Hongqi XinFrom the Department of Neurology, Henry Ford Hospital, Detroit, MI (H.X., M.K., F.W., J.-Y.Q., X.S.L., M.M.A., B.B., Z.G.Z., M.C.); and Department of Physics, Oakland University, Rochester, MI (M.C.).
Mark KatakowskiFrom the Department of Neurology, Henry Ford Hospital, Detroit, MI (H.X., M.K., F.W., J.-Y.Q., X.S.L., M.M.A., B.B., Z.G.Z., M.C.); and Department of Physics, Oakland University, Rochester, MI (M.C.).
Fengjie WangFrom the Department of Neurology, Henry Ford Hospital, Detroit, MI (H.X., M.K., F.W., J.-Y.Q., X.S.L., M.M.A., B.B., Z.G.Z., M.C.); and Department of Physics, Oakland University, Rochester, MI (M.C.).
Jian-Yong QianFrom the Department of Neurology, Henry Ford Hospital, Detroit, MI (H.X., M.K., F.W., J.-Y.Q., X.S.L., M.M.A., B.B., Z.G.Z., M.C.); and Department of Physics, Oakland University, Rochester, MI (M.C.).
Xian Shuang LiuFrom the Department of Neurology, Henry Ford Hospital, Detroit, MI (H.X., M.K., F.W., J.-Y.Q., X.S.L., M.M.A., B.B., Z.G.Z., M.C.); and Department of Physics, Oakland University, Rochester, MI (M.C.).
Meser M AliFrom the Department of Neurology, Henry Ford Hospital, Detroit, MI (H.X., M.K., F.W., J.-Y.Q., X.S.L., M.M.A., B.B., Z.G.Z., M.C.); and Department of Physics, Oakland University, Rochester, MI (M.C.).
Benjamin BullerFrom the Department of Neurology, Henry Ford Hospital, Detroit, MI (H.X., M.K., F.W., J.-Y.Q., X.S.L., M.M.A., B.B., Z.G.Z., M.C.); and Department of Physics, Oakland University, Rochester, MI (M.C.).
Zheng Gang ZhangFrom the Department of Neurology, Henry Ford Hospital, Detroit, MI (H.X., M.K., F.W., J.-Y.Q., X.S.L., M.M.A., B.B., Z.G.Z., M.C.); and Department of Physics, Oakland University, Rochester, MI (M.C.).
Michael ChoppFrom the Department of Neurology, Henry Ford Hospital, Detroit, MI (H.X., M.K., F.W., J.-Y.Q., X.S.L., M.M.A., B.B., Z.G.Z., M.C.); and Department of Physics, Oakland University, Rochester, MI (M.C.). mchopp1@hfhs.org.
Oakland University · US

Funding

miR-17-92 exosome treatment of strokeR01NS088656 · NINDS · HENRY FORD HEALTH SYSTEM · PI CHOPP, MICHAEL · 2015 to 2019
$1.6M
MicroRNAs and neurogenesis after strokeR01NS075156 · NINDS · HENRY FORD HEALTH SYSTEM · PI ZHANG, ZHENG GANG · 2011 to 2015
$1.6M
Exosome transfer of miR-133b mediates MSC induced neurological recovery after strR01NS081189 · NINDS · HENRY FORD HEALTH SYSTEM · PI XIN, HONGQI · 2013 to 2016
$1.2M
NINDS NIH HHS R01 NS075156NINDS NIH HHS R01 NS081189NINDS NIH HHS R01 NS088656
6 · The paper itself

Abstract

background and purposeMultipotent mesenchymal stromal cell (MSC) harvested exosomes are hypothesized as the major paracrine effectors of MSCs. In vitro, the miR-17-92 cluster promotes oligodendrogenesis, neurogenesis, and axonal outgrowth. We, therefore, investigated whether the miR-17-92 cluster-enriched exosomes harvested from MSCs transfected with an miR-17-92 cluster plasmid enhance neurological recovery compared with control MSC-derived exosomes.

methodsRats subjected to 2 hours of transient middle cerebral artery occlusion were intravenously administered miR-17-92 cluster-enriched exosomes, control MSC exosomes, or liposomes and were euthanized 28 days post-middle cerebral artery occlusion. Histochemistry, immunohistochemistry, and Golgi-Cox staining were used to assess dendritic, axonal, synaptic, and myelin remodeling. Expression of phosphatase and tensin homolog and activation of its downstream proteins, protein kinase B, mechanistic target of rapamycin, and glycogen synthase kinase 3β in the peri-infarct region were measured by means of Western blots.

resultsCompared with the liposome treatment, both exosome treatment groups exhibited significant improvement of functional recovery, but miR-17-92 cluster-enriched exosome treatment had significantly more robust effects on improvement of neurological function and enhancements of oligodendrogenesis, neurogenesis, and neurite remodeling/neuronal dendrite plasticity in the ischemic boundary zone (IBZ) than the control MSC exosome treatment. Moreover, miR-17-92 cluster-enriched exosome treatment substantially inhibited phosphatase and tensin homolog, a validated miR-17-92 cluster target gene, and subsequently increased the phosphorylation of phosphatase and tensin homolog downstream proteins, protein kinase B, mechanistic target of rapamycin, and glycogen synthase kinase 3β compared with control MSC exosome treatment.

conclusionsOur data suggest that treatment of stroke with tailored exosomes enriched with the miR-17-92 cluster increases neural plasticity and functional recovery after stroke, possibly via targeting phosphatase and tensin homolog to activate the PI3K/protein kinase B/mechanistic target of rapamycin/glycogen synthase kinase 3β signaling pathway.

Indexed as

Multigene FamilyNeuronal PlasticityAnimalsCells, CulturedDisease Models, AnimalExosomesMaleMesenchymal Stem CellsMicroRNAsMyelin SheathNeurogenesisNeuronsPhosphatidylinositol 3-KinasesRats, WistarRecovery of FunctionStrokeMicroRNAsPhosphatidylinositol 3-Kinasesexosomefunctional recoverymiR-17–92 clusterneural plasticityneurogenesisoligodendrogenesisstroke

Identifiers

PMID28232590
PMCPMC5330787
OpenAlexW2590617978

What OpenQuestion holds

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Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.