ArticleMicrobial cell factories2017
Ethylene production with engineered Synechocystis sp PCC 6803 strains.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 63 citations in OpenAlex.
- Cyanobacterial conversion of COChemical science · 2026Review
- Metabolic engineering of Synechocystis sp. PCC 6803 for olivetolic acid production.Microbial cell factories · 2026Article
- Enhanced Production of High-Value Porphyrin Compound Heme by Metabolic Engineering Modification and Mixotrophic Cultivation ofMarine drugs · 2024Article
- Optimal energy and redox metabolism in the cyanobacterium Synechocystis sp. PCC 6803.NPJ systems biology and applications · 2023Article
- Promoting Heme and Phycocyanin Biosynthesis inMarine drugs · 2023Article
- An Alternative Exploitation ofMolecules (Basel, Switzerland) · 2023Article
- Light-Driven Synthetic Biology: Progress in Research and Industrialization of Cyanobacterial Cell Factory.Life (Basel, Switzerland) · 2022Review
- Heterologous Lactate Synthesis inApplied and environmental microbiology · 2022Article
- Construction of an artificial consortium ofFrontiers in microbiology · 2022Article
- Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.Genes · 2021Review
- Photosynthetic Co-Production of Succinate and Ethylene in A Fast-Growing Cyanobacterium,Metabolites · 2020Article
- Lethality caused by ADP-glucose accumulation is suppressed by salt-induced carbon flux redirection in cyanobacteria.Journal of experimental botany · 2020Article
- Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacteriumBiotechnology for biofuels · 2020Article
- Enhanced stable production of ethylene in photosynthetic cyanobacterium Synechococcus elongatus PCC 7942.World journal of microbiology & biotechnology · 2019Article
- Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase.Scientific reports · 2019Article
- The effect of modulating the quantity of enzymes in a model ethanol pathway on metabolic flux inPeerJ · 2019Article
- Improvement of L-ornithine production by attenuation of argF in engineered Corynebacterium glutamicum S9114.AMB Express · 2018Article
- Bio-production of gaseous alkenes: ethylene, isoprene, isobutene.Biotechnology for biofuels · 2018Review
- Structures and Mechanisms of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme: Substrate Binding Creates a Twist.Journal of the American Chemical Society · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMetabolic engineering and synthetic biology of cyanobacteria offer a promising sustainable alternative approach for fossil-based ethylene production, by using sunlight via oxygenic photosynthesis, to convert carbon dioxide directly into ethylene. Towards this, both well-studied cyanobacteria, i.e., Synechocystis sp PCC 6803 and Synechococcus elongatus PCC 7942, have been engineered to produce ethylene by introducing the ethylene-forming enzyme (Efe) from Pseudomonas syringae pv. phaseolicola PK2 (the Kudzu strain), which catalyzes the conversion of the ubiquitous tricarboxylic acid cycle intermediate 2-oxoglutarate into ethylene.
resultsThis study focuses on Synechocystis sp PCC 6803 and shows stable ethylene production through the integration of a codon-optimized version of the efe gene under control of the Ptrc promoter and the core Shine-Dalgarno sequence (5'-AGGAGG-3') as the ribosome-binding site (RBS), at the slr0168 neutral site. We have increased ethylene production twofold by RBS screening and further investigated improving ethylene production from a single gene copy of efe, using multiple tandem promoters and by putting our best construct on an RSF1010-based broad-host-self-replicating plasmid, which has a higher copy number than the genome. Moreover, to raise the intracellular amounts of the key Efe substrate, 2-oxoglutarate, from which ethylene is formed, we constructed a glycogen-synthesis knockout mutant (ΔglgC) and introduced the ethylene biosynthetic pathway in it. Under nitrogen limiting conditions, the glycogen knockout strain has increased intracellular 2-oxoglutarate levels; however, surprisingly, ethylene production was lower in this strain than in the wild-type background.
conclusionMaking use of different RBS sequences, production of ethylene ranging over a 20-fold difference has been achieved. However, a further increase of production through multiple tandem promoters and a broad-host plasmid was not achieved speculating that the transcription strength and the gene copy number are not the limiting factors in our system.
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