ArticleChembiochem : a European journal of chemical biology2017
Metabolic Chemical Reporters of Glycans Exhibit Cell-Type-Selective Metabolism and Glycoprotein Labeling.
Article in Chembiochem : a European journal of chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Design rules for bioorthogonal glycan-based tools.The Biochemical journal · 2026Review
- Iterative Bump-and-Hole Engineering Creates a Bioorthogonal Reporter forJournal of the American Chemical Society · 2026Article
- Xylosyltransferase engineering to manipulate proteoglycans in mammalian cells.Nature chemical biology · 2026Article
- ChIP happens: from biochemical origins to the modern omics toolbox for understanding steroid hormone receptors.The Biochemical journal · 2026Review
- GlycoRNA research: from unknown unknowns to known unknowns.Protein & cell · 2026Review
- Iterative Bump-and-hole engineering creates a bioorthogonal reporter forbioRxiv : the preprint server for biology · 2026Article
- Chemoenzymatic Synthesis and Purification of Bioorthogonally Tagged UDP-GlcNAc and UDP-GalNAc Analogues.Current protocols · 2025Article
- Article
- Selective bioorthogonal probe for N-glycan hybrid structures.Nature chemical biology · 2025Article
- FRET imaging of glycoRNA on small extracellular vesicles enabling sensitive cancer diagnostics.Nature communications · 2025Article
- A Bioorthogonal Precision Tool for HumanJournal of the American Chemical Society · 2024Article
- The modified RNA base acpCell · 2024Article
- Selenium-based metabolic oligosaccharide engineering strategy for quantitative glycan detection.Nature communications · 2023Article
- Ribonucleosides from tRNA in hyperglycemic mammalian cells and diabetic murine cardiac models.Life sciences · 2023Article
- Recombinant mucin biotechnology and engineering.Advanced drug delivery reviews · 2023Review
- Cell-type-specific labeling and profiling of glycans in living mice.Nature chemical biology · 2022Article
- Optimization of Metabolic Oligosaccharide Engineering with AcACS chemical biology · 2021Article
- Anomeric Fatty Acid Functionalization Prevents NonenzymaticACS chemical biology · 2021Article
- Chemical reporters to study mammalian O-glycosylation.Biochemical Society transactions · 2021Review
- Synthesis of selected unnatural sugar nucleotides for biotechnological applications.Critical reviews in biotechnology · 2021Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Since the pioneering work by Reutter and co-workers that demonstrated structural flexibility in the carbohydrate biosynthesis and glycosylation pathways, many different labs have used unnatural monosaccharide analogues to perform glycan engineering on the surface of living cells. A subset of these unnatural monosaccharides contain bioorthogonal groups that enable the selective installation of visualization or enrichment tags. These metabolic chemical reporters (MCRs) have proven to be powerful for the unbiased identification of glycoproteins; however, they do have certain limitations. For example, they are incorporated substoichiometrically into glycans, and most MCRs are not selective for one class (e.g., O-GlcNAcylation) of glycoprotein. Here, we explore the relationship between the biosynthesis of MCR donor sugars in cells and the labeling levels of four different N-acetylglucosamine- and N-acetylgalactosamine-based MCRs. We found that the buildup of the different donor sugars correlated well with the overall labeling levels but less so with intracellular labeling of proteins by O-GlcNAcylation.
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Registered trials
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