ArticleRadiation research2017
RABiT-II: Implementation of a High-Throughput Micronucleus Biodosimetry Assay on Commercial Biotech Robotic Systems.
Article in Radiation research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Centers for Medical Countermeasures against Radiation Consortium: Past, Present, and Beyond.Radiation research · 2025Review
- Multiwell-based G0-PCC assay for radiation biodosimetry.Scientific reports · 2024Article
- RABiT-III: an Automated Micronucleus Assay at a Non-Specialized Biodosimetry Facility.Radiation research · 2024Article
- High Resolution and Automatable Cytogenetic Biodosimetry Using In Situ Telomere and Centromere Hybridization for the Accurate Detection of DNA Damage: An Overview.International journal of molecular sciences · 2023Review
- A machine learning method for improving the accuracy of radiation biodosimetry by combining data from the dicentric chromosomes and micronucleus assays.Scientific reports · 2022Article
- Rapid and automatic detection of micronuclei in binucleated lymphocytes image.Scientific reports · 2022Article
- Article
- Transportation container for pre-processing cytogenetic assays in radiation accidents.Scientific reports · 2021Article
- Automation and data-driven design of polymer therapeutics.Advanced drug delivery reviews · 2021Review
- Machine learning methodology for high throughput personalized neutron dose reconstruction in mixed neutron + photon exposures.Scientific reports · 2021Article
- Assessment of absorbed dose of gamma rays using the simultaneous determination of inactive hemoglobin derivatives as a biological dosimeter.Radiation and environmental biophysics · 2020Article
- The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis.Journal of radiation research · 2020Article
- Article
- Transcriptomic responses in mouse blood during the first week after in vivo gamma irradiation.Scientific reports · 2019Article
- RABiT-II-DCA: A Fully-automated Dicentric Chromosome Assay in Multiwell Plates.Radiation research · 2019Article
- Saving normal tissues - a goal for the ages.International journal of radiation biology · 2019Review
- Effect of dose and dose rate on temporal γ-H2AX kinetics in mouse blood and spleen mononuclear cells in vivo following Cesium-137 administration.BMC molecular and cell biology · 2019Article
- Automated Triage Radiation Biodosimetry: Integrating Imaging Flow Cytometry with High-Throughput Robotics to Perform the Cytokinesis-Block Micronucleus Assay.Radiation research · 2019Article
- RABiT-II: A Fully-Automated Micronucleus Assay System with Shortened Time to Result.Radiation research · 2019Article
- Development of electronic training and telescoring tools to increase the surge capacity of dicentric chromosome scorers for radiological/nuclear mass casualty incidents.Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We demonstrate the use of high-throughput biodosimetry platforms based on commercial high-throughput/high-content screening robotic systems. The cytokinesis-block micronucleus (CBMN) assay, using only 20 μl whole blood from a fingerstick, was implemented on a PerkinElmer cell::explorer and General Electric IN Cell Analyzer 2000. On average 500 binucleated cells per sample were detected by our FluorQuantMN software. A calibration curve was generated in the radiation dose range up to 5.0 Gy using the data from 8 donors and 48,083 binucleated cells in total. The study described here demonstrates that high-throughput radiation biodosimetry is practical using current commercial high-throughput/high-content screening robotic systems, which can be readily programmed to perform and analyze robotics-optimized cytogenetic assays. Application to other commercial high-throughput/high-content screening systems beyond the ones used in this study is clearly practical. This approach will allow much wider access to high-throughput biodosimetric screening for large-scale radiological incidents than is currently available.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.