ArticleScientific reports2017
Potential microbial contamination during sampling of permafrost soil assessed by tracers.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Exploring the biosynthesis potential of permafrost microbiomes.Environmental microbiome · 2024Article
- A Critical Assessment of the Congruency between Environmental DNA and Palaeoecology for the Biodiversity Monitoring and Palaeoenvironmental Reconstruction.International journal of environmental research and public health · 2022Review
- Microbial Community Changes in 26,500-Year-Old Thawing Permafrost.Frontiers in microbiology · 2022Article
- Spatially resolved correlative microscopy and microbial identification reveal dynamic depth- and mineral-dependent anabolic activity in salt marsh sediment.Environmental microbiology · 2021Article
- Application of wood ash leads to strong vertical gradients in soil pH changing prokaryotic community structure in forest top soil.Scientific reports · 2021Article
- Optimization of subsampling, decontamination, and DNA extraction of difficult peat and silt permafrost samples.Scientific reports · 2020Article
- Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost.FEMS microbiology ecology · 2020Article
- Methanogenic response to long-term permafrost thaw is determined by paleoenvironment.FEMS microbiology ecology · 2020Article
- Metagenomes from Late Pleistocene Ice Complex Sediments of the Siberian Arctic.Microbiology resource announcements · 2019Article
- Bacterial and protozoan dynamics upon thawing and freezing of an active layer permafrost soil.The ISME journal · 2019Article
- Potential effects of ultraviolet radiation reduction on tundra nitrous oxide and methane fluxes in maritime Antarctica.Scientific reports · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drilling and handling of permanently frozen soil cores without microbial contamination is of concern because contamination e.g. from the active layer above may lead to incorrect interpretation of results in experiments investigating potential and actual microbial activity in these low microbial biomass environments. Here, we present an example of how microbial contamination from active layer soil affected analysis of the potentially active microbial community in permafrost soil. We also present the development and use of two tracers: (1) fluorescent plastic microspheres and (2) Pseudomonas putida genetically tagged with Green Fluorescent Protein production to mimic potential microbial contamination of two permafrost cores. A protocol with special emphasis on avoiding microbial contamination was developed and employed to examine how far microbial contamination can penetrate into permafrost cores. The quantity of tracer elements decreased with depth into the permafrost cores, but the tracers were detected as far as 17 mm from the surface of the cores. The results emphasize that caution should be taken to avoid microbial contamination of permafrost cores and that the application of tracers represents a useful tool to assess penetration of potential microbial contamination into permafrost cores.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.