ArticleScientific reports2017
A novel approach to improve poly-γ-glutamic acid production by NADPH Regeneration in Bacillus licheniformis WX-02.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 84 citations in OpenAlex.
- Recent advances in metabolic engineering for microbial production of L-tyrosine.World journal of microbiology & biotechnology · 2026Review
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- Enhanced Poly-γ-Glutamic Acid Production by a Newly Isolated Bacillus halotolerans F29.Journal of microbiology (Seoul, Korea) · 2024Article
- Electrofermentation increases concentration of poly γ-glutamic acid in Bacillus subtilis biofilms.Microbial biotechnology · 2024Article
- Optimization of trans-4-hydroxyproline synthesis pathway by rearrangement center carbon metabolism in Escherichia coli.Microbial cell factories · 2023Article
- Construct a synthetic Entner-Doudoroff pathway in Bacillus licheniformis for enhancing lichenysin production.World journal of microbiology & biotechnology · 2023Article
- Poly-gamma-glutamic acid biopolymer: a sleeping giant with diverse applications and unique opportunities for commercialization.Biomass conversion and biorefinery · 2023Review
- Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ-glutamic acid production from citric acid.Microbial cell factories · 2022Article
- Bacillus amyloliquefaciens CU33 fermented feather meal-soybean meal product improves the intestinal morphology to promote the growth performance of broilers.Poultry science · 2022Article
- Genetic and metabolic engineering for poly-γ-glutamic acid production: current progress, challenges, and prospects.World journal of microbiology & biotechnology · 2022Review
- Enhanced production of poly-γ-glutamic acid via optimizing the expression cassette ofSynthetic and systems biotechnology · 2022Article
- Metagenomic-Metabolomic Mining ofFrontiers in microbiology · 2022Article
- Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis.Frontiers in bioengineering and biotechnology · 2022Review
- Identification and Characterization of a Novel Soluble Pyridine Nucleotide Transhydrogenase from Streptomyces avermitilis.Current microbiology · 2021Article
- Efficient One-Step Biocatalytic Multienzyme Cascade Strategy for Direct Conversion of Phytosterol to C-17-Hydroxylated Steroids.Applied and environmental microbiology · 2021Article
- Metabolic Engineering of Central Carbon Metabolism of Bacillus licheniformis for Enhanced Production of Poly-γ-glutamic Acid.Applied biochemistry and biotechnology · 2021Article
- Positioning Bacillus subtilis as terpenoid cell factory.Journal of applied microbiology · 2021Review
- Study on the mechanism of production of γ-PGA and nattokinase in Bacillus subtilis natto based on RNA-seq analysis.Microbial cell factories · 2021Article
- Biomedical Applications of Bacteria-Derived Polymers.Polymers · 2021Review
- Systematic engineering of branch chain amino acid supply modules for the enhanced production of bacitracin fromMetabolic engineering communications · 2020Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poly-γ-glutamic acid (γ-PGA) is an important biochemical product with a variety of applications. This work reports a novel approach to improve γ-PGA through over expression of key enzymes in cofactor NADPH generating process for NADPH pool. Six genes encoding the key enzymes in NADPH generation were over-expressed in the γ-PGA producing strain B. licheniformis WX-02. Among various recombinants, the strain over-expressing zwf gene (coding for glucose-6-phosphate dehydrogenase), WX-zwf, produced the highest γ-PGA concentration (9.13 g/L), 35% improvement compared to the control strain WX-pHY300. However, the growth rates and glucose uptake rates of the mutant WX-zwf were decreased. The transcriptional levels of the genes pgsB and pgsC responsible for γ-PGA biosynthesis were increased by 8.21- and 5.26-fold, respectively. The Zwf activity of the zwf over expression strain increased by 9.28-fold, which led to the improvement of the NADPH generation, and decrease of accumulation of by-products acetoin and 2,3-butanediol. Collectively, these results demonstrated that NADPH generation via over-expression of Zwf is as an effective strategy to improve the γ-PGA production in B. licheniformis.
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