ArticleMolecular biology of the cell2017
Contact guidance requires spatial control of leading-edge protrusion.
Article in Molecular biology of the cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
47 citing papers in PubMed, 84 citations in OpenAlex.
- Melanoma/CSPG4-Enhanced Collagen-Mediated Contact Guidance Requires Mutant Active BRAF and the CSPG4 Core Protein Cytoplasmic Domain.Cellular and molecular bioengineering · 2026Article
- Formins and Arp2/3 Reciprocally Regulate Contact Guidance on Aligned Collagen Fibrils.Cellular and molecular bioengineering · 2025Article
- Engineering Extracellular Microenvironments: The Impact of Fibrous Materials on Cell Behavior.Advanced healthcare materials · 2025Review
- Mechanochemical waves in focal adhesions during cell migration.Science advances · 2025Article
- Cellular Responses to Nanoscale Topography Mediated Through the RhoA/ROCK Pathway.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Aligned Collagen Fibers Drive Distinct Traction Force Signatures to Regulate Contact Guidance.ACS nano · 2025Article
- A Simple Framework for Agent-Based Modeling with Extracellular Matrix.Bulletin of mathematical biology · 2025Article
- The influence of physical and spatial substrate characteristics on endothelial cells.Materials today. Bio · 2024Review
- Microengineering 3D Collagen Matrices with Tumor-Mimetic Gradients in Fiber Alignment.Advanced functional materials · 2024Article
- Highly oriented hydrogels for tissue regeneration: design strategies, cellular mechanisms, and biomedical applications.Theranostics · 2024Review
- 3D printed zirconia used as dental materials: a critical review.Journal of biological engineering · 2023Review
- Dynein-Powered Cell Locomotion Guides Metastasis of Breast Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- A noncommutative combinatorial protein logic circuit controls cell orientation in nanoenvironments.Science advances · 2023Article
- Accelerated epithelial layer healing induced by tactile anisotropy in surface topography.Science advances · 2023Article
- Dynein-Powered Cell Locomotion Guides Metastasis of Breast Cancer.bioRxiv : the preprint server for biology · 2023Article
- Spatial Gradients of E-Cadherin and Fibronectin in TGF-β1-Treated Epithelial Colonies Are Independent of Fibronectin Fibril Assembly.International journal of molecular sciences · 2023Article
- Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance.Materials today. Bio · 2023Article
- Elucidating the signal for contact guidance contained in aligned fibrils with a microstructural-mechanical model.Journal of the Royal Society, Interface · 2022Article
- Chronic lung diseases: entangled in extracellular matrix.European respiratory review : an official journal of the European Respiratory Society · 2022Review
- Patterned photocrosslinking to establish stiffness anisotropies in fibrous 3D hydrogels.Acta biomaterialia · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In vivo, geometric cues from the extracellular matrix (ECM) are critical for the regulation of cell shape, adhesion, and migration. During contact guidance, the fibrillar architecture of the ECM promotes an elongated cell shape and migration along the fibrils. The subcellular mechanisms by which cells sense ECM geometry and translate it into changes in shape and migration direction are not understood. Here we pattern linear fibronectin features to mimic fibrillar ECM and elucidate the mechanisms of contact guidance. By systematically varying patterned line spacing, we show that a 2-μm spacing is sufficient to promote cell shape elongation and migration parallel to the ECM, or contact guidance. As line spacing is increased, contact guidance increases without affecting migration speed. To elucidate the subcellular mechanisms of contact guidance, we analyze quantitatively protrusion dynamics and find that the structured ECM orients cellular protrusions parallel to the ECM. This spatial organization of protrusion relies on myosin II contractility, and feedback between adhesion and Rac-mediated protrusive activity, such that we find Arp2/3 inhibition can promote contact guidance. Together our data support a model for contact guidance in which the ECM enforces spatial constraints on the lamellipodia that result in cell shape elongation and enforce migration direction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.