ArticleThe Histochemical journal
A new histochemical method for the selective periodate oxidation of total tissue sialic acids.
Article in The Histochemical journal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 57 citations in OpenAlex.
- Morphological and histochemical characteristics of the epithelium of ovarian lamellae of Genypterus blacodes (Schneider, 1801).Fish physiology and biochemistry · 2009Article
- Histochemical alterations of mucin in normal colon, inflammatory bowel disease and colonic adenocarcinoma.The Histochemical journal · 1995Article
- Article
- How selective are the 'selective periodate oxidation' methods used in sialic acid histochemistry?The Histochemical journal · 1991Article
- Histochemical identification of 9-O-acyl sialic acids: studies of bovine submandibular and rat sublingual gland and human colon.The Histochemical journal · 1991Article
- Histochemical classification based upon reaction types and its application to carbohydrate histochemistry.The Histochemical journal · 1990Review
- Histochemical studies of intestinal epithelial goblet cell glycoproteins during the development of the human foetus.The Histochemical journal · 1990Article
- The histochemical specificity of high iron diamine-alcian blue.The Histochemical journal · 1989Article
- Application of the selective periodate oxidation of sialic acids. III. Identification of neuraminidase-sensitive and neuraminidase-resistant sialic acids and their side chain O-acyl variants.The Histochemical journal · 1988Article
- Histochemical studies of the colonic epithelial glycoproteins of the normal rabbit.The Histochemical journal · 1988Article
- A new method for the histochemical demonstration of O-acyl sugars in human colonic epithelial glycoproteins.The Histochemical journal · 1988Article
- Histochemical procedures for the simultaneous visualization of neutral sugars and either sialic acid and its O-acyl variants or O-sulphate ester. II. Methods based upon the periodic acid-phenylhydrazine-Schiff reaction.The Histochemical journal · 1987Article
- Histochemical procedures for the simultaneous visualization of neutral sugars and either sialic acid and its side chain O-acyl variants or O-sulphate ester. I. Methods based upon the selective periodate oxidation of sialic acids.The Histochemical journal · 1987Article
- Methods for the identification of side chain O-acyl substituted sialic acids and for the simultaneous visualization of sialic acid, its side chain O-acyl variants and O-sulphate ester.The Histochemical journalArticle
- Histochemical studies of epithelial cell glycoproteins in normal rat colon.The Histochemical journalArticle
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Evaluation of the intensity of the periodic acid-Schiff (PAS) staining produced following oxidation for 1 h at 4 degrees C with 0.4 mM periodic acid in approximately 1 M hydrochloric acid indicated that this reagent completely oxidized all available sialic acid residues of either the sialo- or sialosulphoglycoproteins of human and rat colon or the sialoglycoproteins of rat sublingual gland. These conditions produced no visible Schiff staining of either neutral macromolecules or vicinal diols located on hexose, 6-deoxyhexose or N-acetylhexosamine residues ('neutral sugars') of sialo- and sialosulphoglycoproteins. Furthermore, there was no extraction of epithelial glycoproteins or de-O-acylation of side chain substituted sialic acid residues. These data demonstrate that 0.4 mM periodic acid in approximately 1 M hydrochloric acid can be used as a specific reagent for the selective visualization of sialic acids in the PAS procedure. Studies of the mechanism of the oxidation of neutral sugars with 0.4 mM periodic acid in approximately 1 M hydrochloric acid indicated that their lack of PAS reactivity was not due to the production of Schiff unreactive hemiacetals or hemialdals. It is suggested that the selectivity of 0.4 mM periodic acid in approximately 1 M hydrochloric acid is a result of an increase in the rate of the oxidation of the sialic acid residues together with a decrease in the rate of oxidation of neutral sugars.
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