ArticlePloS one2017
Epileptogenesis in organotypic hippocampal cultures has limited dependence on culture medium composition.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Prolonged Hyperactivity Elicits Massive and Persistent Chloride Ion Redistribution in Subsets of Cultured Hippocampal Dentate Granule Cells.bioRxiv : the preprint server for biology · 2025Article
- The NLRP3 Inflammasome in Neurodegenerative Disorders: Insights from Epileptic Models.Biomedicines · 2023Review
- A Dynamic Balance between Neuronal Death and Clearance in anThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Blockage of STAT3 during epileptogenesis prevents GABAergic loss and imprinting of the epileptic state.Brain : a journal of neurology · 2023Article
- Targeting firing rate neuronal homeostasis can prevent seizures.Disease models & mechanisms · 2022Article
- Ictogenesis? That's Random…..Epilepsy currents · 2022Article
- Lab-on-Chip Microsystems forFrontiers in bioengineering and biotechnology · 2022Review
- Microvascular changes associated with epilepsy: A narrative review.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2021Review
- Variability of seizure-like activity in anHeliyon · 2020Article
- CurrentCurrent opinion in biomedical engineering · 2020Article
- Epilepsy-on-a-Chip System for Antiepileptic Drug Discovery.IEEE transactions on bio-medical engineering · 2019Article
- Trigonelline protects hippocampus against intracerebral Aβ(1-40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms.Metabolic brain disease · 2019Article
- A Proposed Mechanism for Spontaneous Transitions between Interictal and Ictal Activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- How do we use in vitro models to understand epileptiform and ictal activity? A report of the TASK1-WG4 group of the ILAE/AES Joint Translational Task Force.Epilepsia open · 2018Article
- Review
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5 authors.
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Abstract
Rodent organotypic hippocampal cultures spontaneously develop epileptiform activity after approximately 2 weeks in vitro and are increasingly used as a model of chronic post-traumatic epilepsy. However, organotypic cultures are maintained in an artificial environment (culture medium), which contains electrolytes, glucose, amino acids and other components that are not present at the same concentrations in cerebrospinal fluid (CSF). Therefore, it is possible that epileptogenesis in organotypic cultures is driven by these components. We examined the influence of medium composition on epileptogenesis. Epileptogenesis was evaluated by measurements of lactate and lactate dehydrogenase (LDH) levels (biomarkers of ictal activity and cell death, respectively) in spent culture media, immunohistochemistry and automated 3-D cell counts, and extracellular recordings from CA3 regions. Changes in culture medium components moderately influenced lactate and LDH levels as well as electrographic seizure burden and cell death. However, epileptogenesis occurred in any culture medium that was capable of supporting neural survival. We conclude that medium composition is unlikely to be the cause of epileptogenesis in the organotypic hippocampal culture model of chronic post-traumatic epilepsy.
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