ArticlePloS one2017
Use of bovine recombinant prion protein and real-time quaking-induced conversion to detect cattle transmissible mink encephalopathy prions and discriminate classical and atypical L- and H-Type bovine spongiform encephalopathy.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Practical Real-Time Quaking-Induced Conversion for Detecting Classical Bovine Spongiform Encephalopathy and Classical and Atypical Scrapie Prions.Pathogens (Basel, Switzerland) · 2026Article
- RT-QuIC as ultrasensitive method for prion detection.Cell and tissue research · 2023Review
- Standardization of Data Analysis for RT-QuIC-Based Detection of Chronic Wasting Disease.Pathogens (Basel, Switzerland) · 2023Article
- A field-deployable diagnostic assay for the visual detection of misfolded prions.Scientific reports · 2022Article
- Hofmeister Effect in RT-QuIC Seeding Activity of Chronic Wasting Disease Prions.Frontiers in bioengineering and biotechnology · 2021Article
- Real-Time Quaking-Induced Conversion Detection of PrPFrontiers in veterinary science · 2021Article
- Novel Strain of the Chronic Wasting Disease Agent Isolated From Experimentally Inoculated Elk With LL132 Prion Protein.Scientific reports · 2020Article
- Role of donor genotype in RT-QuIC seeding activity of chronic wasting disease prions using human and bank vole substrates.PloS one · 2020Article
- Evaluation of Antemortem Diagnostic Techniques in Goats Naturally Infected With Scrapie.Frontiers in veterinary science · 2020Article
- PAD-Beads enrichment enhances detection of PrPBMC research notes · 2019Article
- Early and Pre-Clinical Detection of Prion Seeding Activity in Cerebrospinal Fluid of Goats using Real-Time Quaking-Induced Conversion Assay.Scientific reports · 2019Article
- Preparation of lyophilized recombinant prion protein for TSE diagnosis by RT-QuIC.BMC research notes · 2018Article
- Leishmaniasis and glycosaminoglycans: a future therapeutic strategy?Parasites & vectors · 2018Review
- Pathologic and biochemical characterization of PrPBMC veterinary research · 2018Article
- Differential effects of divalent cations on elk prion protein fibril formation and stability.Prion · 2018Article
- Source genotype influence on cross species transmission of transmissible spongiform encephalopathies evaluated by RT-QuIC.PloS one · 2018Article
- Real-Time Quaking-Induced Conversion Detection of Bovine Spongiform Encephalopathy Prions in a Subclinical Steer.Frontiers in veterinary science · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prions are amyloid-forming proteins that cause transmissible spongiform encephalopathies through a process involving conversion from the normal cellular prion protein to the pathogenic misfolded conformation (PrPSc). This conversion has been used for in vitro assays including serial protein misfolding amplification and real-time quaking induced conversion (RT-QuIC). RT-QuIC can be used for the detection of prions in a variety of biological tissues from humans and animals. Extensive work has been done to demonstrate that RT-QuIC is a rapid, specific, and highly sensitive prion detection assay. RT-QuIC uses recombinant prion protein to detect minute amounts of PrPSc. RT-QuIC has been successfully used to detect PrPSc from different prion diseases with a variety of substrates including hamster, human, sheep, bank vole, bovine and chimeric forms of prion protein. However, recombinant bovine prion protein has not been used to detect transmissible mink encephalopathy (TME) or to differentiate types of bovine spongiform encephalopathy (BSE) in samples from cattle. We evaluated whether PrPSc from TME and BSE infected cattle can be detected with RT-QuIC using recombinant bovine prion proteins, and optimized the reaction conditions to specifically detect cattle TME and to discriminate between classical and atypical BSE by conversion efficiency. We also found that substrate composed of the disease associated E211K mutant protein can be effective for the detection of TME in cattle and that wild type prion protein appears to be a practical substrate to discriminate between the different types of BSEs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.