ArticlePloS one2017
Combined small RNA and degradome sequencing reveals complex microRNA regulation of catechin biosynthesis in tea (Camellia sinensis).
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Changes in the expression of microRNAs regulate podophyllotoxin accumulation in Linum album cells to phenylalanine.BMC plant biology · 2026Article
- Comprehensive microRNA analysis toward exploring a new functional component in Matcha green tea.Food chemistry. Molecular sciences · 2025Article
- Exploring pharmacological potential ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- gma-miR828a Negatively Regulates Resistance to Tea Leaf Spot Caused by Lasiodiplodia theobromae Through Targeting the CsMYB28-CsRPP13 Module.Molecular plant pathology · 2025Article
- Fine-tuning plant valuable secondary metabolite biosynthesis via small RNA manipulation: strategies and potential.Planta · 2024Review
- Light-Dependent Regulatory Interactions between the Redox System and miRNAs and Their Biochemical and Physiological Effects in Plants.International journal of molecular sciences · 2023Review
- Anthocyanin regulatory networks in Solanum tuberosum L. leaves elucidated via integrated metabolomics, transcriptomics, and StAN1 overexpression.BMC plant biology · 2022Article
- Exploring the Effect of Methyl Jasmonate on the Expression of microRNAs Involved in Biosynthesis of Active Compounds of Rosemary Cell Suspension Cultures through RNA-Sequencing.International journal of molecular sciences · 2022Article
- Biosynthesis of Secondary Metabolites Based on the Regulation of MicroRNAs.BioMed research international · 2022Review
- Genome-wide identification, evolutionary relationship and expression analysis of AGO, DCL and RDR family genes in tea.Scientific reports · 2021Article
- Genome-Wide Identification and Expression Patterns of the C2H2-Zinc Finger Gene Family Related to Stress Responses and Catechins Accumulation inInternational journal of molecular sciences · 2021Article
- Article
- Omics Technologies to Enhance Plant Based Functional Foods: An Overview.Frontiers in genetics · 2021Review
- Utilization of microRNAs and their regulatory functions for improving biotic stress tolerance in tea plant [RNA biology · 2020Article
- Tea plant genomics: achievements, challenges and perspectives.Horticulture research · 2020Review
- MicroRNAs and their targeted genes associated with phase changes of stem explants during tissue culture of tea plant.Scientific reports · 2019Article
- Article
- ConservedInternational journal of molecular sciences · 2019Article
- miR394 Acts as a Negative Regulator ofFrontiers in plant science · 2018Article
- Effects of blue light on flavonoid accumulation linked to the expression of miR393, miR394 and miR395 in longan embryogenic calli.PloS one · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs are endogenous non-coding small RNAs playing crucial regulatory roles in plants. Tea, a globally popular non-alcoholic drink, is rich in health-enhancing catechins. In this study, 69 conserved and 47 novel miRNAs targeting 644 genes were identified by high-throughout sequencing. Predicted target genes of miRNAs were mainly involved in plant growth, signal transduction, morphogenesis and defense. To further identify targets of tea miRNAs, degradome sequencing and RNA ligase-mediated rapid amplification of 5'cDNA ends (RLM-RACE) were applied. Using degradome sequencing, 26 genes mainly involved in transcription factor, resistance protein and signal transduction protein synthesis were identified as potential miRNA targets, with 5 genes subsequently verified. Quantitative real-time PCR (qRT-PCR) revealed that the expression patterns of novel-miR1, novel-miR2, csn-miR160a, csn-miR162a, csn-miR394 and csn-miR396a were negatively correlated with catechin content. The expression of six miRNAs (csn-miRNA167a, csn-miR2593e, csn-miR4380a, csn-miR3444b, csn-miR5251 and csn-miR7777-5p.1) and their target genes involved in catechin biosynthesis were also analyzed by qRT-PCR. Negative and positive correlations were found between these miRNAs and catechin contents, while positive correlations were found between their target genes and catechin content. This result suggests that these miRNAs may negatively regulate catechin biosynthesis by down-regulating their biosynthesis-related target genes. Taken together, our results indicate that miRNAs are crucial regulators in tea, with the results of 5'-RLM-RACE and expression analyses revealing the important role of miRNAs in catechin anabolism. Our findings should facilitate future research to elucidate the function of miRNAs in catechin biosynthesis.
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