ArticleAnnals of laboratory medicine2017
Comparison of the Bruker Biotyper and VITEK MS Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Systems Using a Formic Acid Extraction Method to Identify Common and Uncommon Yeast Isolates.
Article in Annals of laboratory medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Comparison of Bruker Biotyper Sirius, VITEK MS PRIME, and ASTA MicroIDSys MALDI-TOF MS Systems forAnnals of laboratory medicine · 2026Article
- Application of matrix-assisted laser desorption/ionization time-of flight mass spectrometry in clinical testing and diagnosis.Frontiers in cellular and infection microbiology · 2025Review
- Formic acid sandwich method is well-suited for filamentous fungi identification and improves turn around time using Zybio EXS2600 mass spectrometry.BMC microbiology · 2024Article
- MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology Laboratory.Microorganisms · 2024Review
- Molecular and Clinical Features of Fluconazole Non-susceptibleAnnals of laboratory medicine · 2023Article
- Candida dubliniensis fungemia in a patient with severe COVID-19: A case report.Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2022Article
- Nationwide Surveillance of Antifungal Resistance ofJournal of fungi (Basel, Switzerland) · 2022Article
- "Omic" Approaches to Bacteria and Antibiotic Resistance Identification.International journal of molecular sciences · 2022Review
- Mass spectrometry in research laboratories and clinical diagnostic: a new era in medical mycology.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Review
- Re-classification ofiScience · 2021Article
- Disk Diffusion Susceptibility Testing for the Rapid Detection of Fluconazole Resistance inAnnals of laboratory medicine · 2021Article
- Matrix-Assisted Laser Desorption Ionization Time-of-Flight for Fungal Identification.Clinics in laboratory medicine · 2021Review
- Evaluation of three sample preparation methods for the identification of clinical strains by using two MALDI-TOF MS systems.Journal of mass spectrometry : JMS · 2021Article
- Identification ofFrontiers in cellular and infection microbiology · 2021Article
- Yeasts in Liquid Swine Diets: Identification Methods, Growth Temperatures and Gas-Formation Potential.Journal of fungi (Basel, Switzerland) · 2020Article
- Molecular epidemiology of Candida tropicalis isolated from urogenital tract infections.MicrobiologyOpen · 2020Article
- Evaluation of Two Commercial Broth Microdilution Methods Using Different Interpretive Criteria for the Detection of Molecular Mechanisms of Acquired Azole and Echinocandin Resistance in Four CommonAntimicrobial agents and chemotherapy · 2020Article
- Matrix-assisted laser desorption ionization time-of-flight mass spectrometry combined with UF-5000i urine flow cytometry to directly identify pathogens in clinical urine specimens within 1 hour.Annals of translational medicine · 2020Article
- Multilocus Sequence Typing Reveals Clonality of Fluconazole-NonsusceptibleFrontiers in microbiology · 2020Article
- Candida auris Clinical Isolates from South Korea: Identification, Antifungal Susceptibility, and Genotyping.Journal of clinical microbiology · 2019Article
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8 authors.
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Abstract
backgroundMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows rapid and accurate identification of clinical yeast isolates. In-tube formic acid/acetonitrile (FA/ACN) extraction is recommended prior to the analysis with MALDI Biotyper, but the direct on-plate FA extraction is simpler. We compared the Biotyper with the VITEK MS for the identification of various clinically relevant yeast species, focusing on the use of the FA extraction method.
methodsWe analyzed 309 clinical isolates of 42 yeast species (four common Candida species, Cryptococcus neoformans, and 37 uncommon yeast species) using the Biotyper and VITEK MS systems. FA extraction was used initially for all isolates. If 'no identification' result was obtained following the initial FA extraction, these samples were then retested by using FA (both systems, additive FA) or FA/ACN (Biotyper only, additive FA/ACN) extraction. These results were compared with those obtained by sequence-based identification.
resultsBoth systems correctly identified all 158 isolates of the four common Candida species after the initial FA extraction. The Biotyper correctly identified 8.7%, 30.4%, and 100% of 23 C. neoformans isolates after performing initial FA, additive FA, and FA/ACN extractions, respectively, while VITEK MS identified all C. neoformans isolates after the initial FA extraction. Both systems had comparable identification rates of 37 uncommon yeast species (128 isolates), following the initial FA (Biotyper, 74.2%; VITEK MS, 73.4%) or additive FA (Biotyper, 82.0%; VITEK MS, 73.4%).
conclusionsThe identification rate of most common and uncommon yeast isolates is comparable between simple FA extraction/Biotyper method and VITEK MS methods, but FA/ACN extraction is necessary for C. neoformans identification by Biotyper.
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