ArticleRNA (New York, N.Y.)2017
Inducing circular RNA formation using the CRISPR endoribonuclease Csy4.
Article in RNA (New York, N.Y.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- A trigger-inducible split-Csy4 architecture for programmable RNA modulation.Nucleic acids research · 2025Article
- Techniques for investigating lncRNA transcript functions in neurodevelopment.Molecular psychiatry · 2024Review
- Circular RNA vaccine in disease prevention and treatment.Signal transduction and targeted therapy · 2023Review
- Transcription-wide impact by RESCUE-induced off-target single-nucleotide variants in mammalian cells.Journal of molecular cell biology · 2023Article
- Circular RNA and intervertebral disc degeneration: unravelling mechanisms and implications.Frontiers in molecular biosciences · 2023Review
- PERSIST platform provides programmable RNA regulation using CRISPR endoRNases.Nature communications · 2022Article
- The design and synthesis of circular RNAs.Methods (San Diego, Calif.) · 2021Review
- Engineering highly efficient backsplicing and translation of synthetic circRNAs.Molecular therapy. Nucleic acids · 2021Article
- Insights Into the Involvement of Circular RNAs in Autoimmune Diseases.Frontiers in immunology · 2021Review
- Recent advances in understanding circular RNAs.F1000Research · 2020Review
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- Circular RNAs: A novel target among non‑coding RNAs with potential roles in malignant tumors (Review).Molecular medicine reports · 2019Review
- Tissue-Dependent Expression and Translation of Circular RNAs with Recombinant AAV Vectors In Vivo.Molecular therapy. Nucleic acids · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Circular RNAs (circRNAs) are highly stable, covalently closed RNAs that are regulated in a spatiotemporal manner and whose functions are largely unknown. These molecules have the potential to be incorporated into engineered systems with broad technological implications. Here we describe a switch for inducing back-splicing of an engineered circRNA that relies on the CRISPR endoribonuclease, Csy4, as an activator of circularization. The endoribonuclease activity and 3' end-stabilizing properties of Csy4 are particularly suited for this task. Coexpression of Csy4 and the circRNA switch allows for the removal of downstream competitive splice sites and stabilization of the 5' cleavage product. This subsequently results in back-splicing of the 5' cleavage product into a circRNA that can translate a reporter protein from an internal ribosomal entry site (IRES). Our platform outlines a straightforward approach toward regulating splicing and could find potential applications in synthetic biology as well as in studying the properties of different circRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.