Trial reportBMC infectious diseases2017
Comparison of methods for identifying causative bacterial microorganisms in presumed acute endophthalmitis: conventional culture, blood culture, and PCR.
Trial report in BMC infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Antibiotic prophylaxis for preventing endophthalmitis after intravitreal injection: a systematic review.Eye (London, England) · 2018Pooled it
- Contribution of 16S rRNA gene PCR to microbiological diagnosis of endophthalmitis: a retrospective clinical cohort study of 71 vitreous and aqueous humor samples.Journal of ophthalmic inflammation and infection · 2026Article
- The evolution of diagnostic microbiology: integrating culture-based methods and genomic advances.PeerJ · 2026Review
- Metagenomic next-generation sequencing: a game-changer in the diagnosis of unique intraocular infections.Eye (London, England) · 2025Article
- Real-TimeACS biomaterials science & engineering · 2025Article
- Insights into Peptidoglycan-Targeting Radiotracers for Imaging Bacterial Infections: Updates, Challenges, and Future Perspectives.ACS infectious diseases · 2024Review
- Management and Prognosis of Acute Post-Cataract Surgery Endophthalmitis: A 10-Year Retrospective Analysis in Eastern China.Antibiotics (Basel, Switzerland) · 2023Article
- The diagnostic utility of nanopore targeted sequencing in suspected endophthalmitis.International ophthalmology · 2023Article
- Validation of a multi-species-specific PCR panel to diagnose patients with suspected postoperative bacterial endophthalmitis.Acta ophthalmologica · 2022Article
- Nanopore techniques as a potent tool in the diagnosis and treatment of endophthalmitis: a literature review.International journal of ophthalmology · 2022Review
- Microbiological diagnosis of endophthalmitis using nanopore targeted sequencing.Clinical & experimental ophthalmology · 2021Article
- Practical Guidance for Clinical Microbiology Laboratories: Diagnosis of Ocular Infections.Clinical microbiology reviews · 2021Review
- Post-intravitreal injection endophthalmitis secondary to Turicella otitidis: a case report.BMC ophthalmology · 2020Article
- Rapid identification of pathogens involved in pediatric osteoarticular infections by multiplex PCR.Annals of translational medicine · 2020Article
- Impact of antibiotic resistance of pathogens and early vitrectomy on the prognosis of infectious endophthalmitis: a 10-year retrospective study.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2019Article
- Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2019Article
- A Novel Detection ofInfection and drug resistance · 2019Article
- Diagnostic performance of blood culture bottles for vitreous culture compared to conventional microbiological cultures in patients with suspected endophthalmitis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Trends in treatment strategies for suspected bacterial endophthalmitis.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2018Article
- Clinical Practice Update: Management of Infectious Endophthalmitis After Intravitreal Anti-VEGF Injection.Journal of vitreoretinal diseasesReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIdentification of bacterial pathogens in endophthalmitis is important to inform antibiotic selection and treatment decisions. Hemoculture bottles and polymerase chain reaction (PCR) analysis have been proposed to offer good detection sensitivity. This study compared the sensitivity and accuracy of a blood culture system, a PCR approach, and conventional culture methods for identification of causative bacteria in cases of acute endophthalmitis.
methodsTwenty-nine patients with a diagnosis of presumed acute bacterial endophthalmitis who underwent vitreous specimen collection at King Chulalongkorn Memorial Hospital were enrolled in this study. Forty-one specimens were collected. Each specimen was divided into three parts, and each part was analyzed using one of three microbial identification techniques: conventional plate culture, blood culture, and polymerase chain reaction and sequencing. The results of the three methods were then compared.
resultsBacteria were identified in 15 of the 41 specimens (36.5%). Five (12.2%) specimens were positive by conventional culture methods, 11 (26.8%) were positive by hemoculture, and 11 (26.8%) were positive by PCR. Cohen's kappa analysis revealed p-values for conventional methods vs. hemoculture, conventional methods vs. PCR, and hemoculture vs. PCR of 0.057, 0.33, and 0.009, respectively. Higher detection rates of Enterococcus faecalis were observed for hemoculture and PCR than for conventional methods.
conclusionsBlood culture bottles and PCR detection may facilitate bacterial identification in cases of presumed acute endophthalmitis. These techniques should be used in addition to conventional plate culture methods because they provide a greater degree of sensitivity than conventional plate culture alone for the detection of specific microorganisms such as E. faecalis.
trial registrationThai Clinical Trial Register No. TCTR20110000024 .
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.