ArticleBMC infectious diseases2017
Field evaluation of a recombinase polymerase amplification assay for the diagnosis of Schistosoma japonicum infection in Hunan province of China.
Article in BMC infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 2 of them syntheses that pooled it.
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Who cites it
27 citing papers in PubMed, 2 syntheses or guidelines pooled it, 59 citations in OpenAlex.
- Diagnostic test accuracy for detecting Schistosoma japonicum and S. mekongi in humans: A systematic review and meta-analysis.PLoS neglected tropical diseases · 2021Pooled it
- An update on non-invasive urine diagnostics for human-infecting parasitic helminths: what more could be done and how?Parasitology · 2020Pooled it
- Optimisation and analytical assessment of a TaqMan™ probe-based real-time PCR assay designed to diagnose infection with Schistosoma japonicum.Parasites & vectors · 2026Article
- A mini review on advances in diagnostic techniques forFrontiers in veterinary science · 2026Review
- Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East Gabon.Diagnostics (Basel, Switzerland) · 2025Article
- Review
- A prognostic model for Schistosoma japonicum infection-associated liver hepatocellular carcinoma: strengthening the connection through initial biological experiments.Infectious agents and cancer · 2024Article
- Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis.iScience · 2023Article
- Molecular diagnosis of urogenital schistosomiasis in pre-school children, school-aged children and women of reproductive age at community level in central Senegal.Parasites & vectors · 2023Article
- Advances in Diagnosis of Schistosomiasis: Focus on Challenges and Future Approaches.International journal of general medicine · 2023Review
- The Efficiency of Commercial Immunodiagnostic Assays for the Field Detection ofPathogens (Basel, Switzerland) · 2022Article
- Laboratory Evaluation of a Basic Recombinase Polymerase Amplification (RPA) Assay for Early Detection ofPathogens (Basel, Switzerland) · 2022Article
- Molecular Techniques as Alternatives of Diagnostic Tools in China as Schistosomiasis Moving towards Elimination.Pathogens (Basel, Switzerland) · 2022Review
- Development of real-time and lateral flow recombinase polymerase amplification assays for rapid detection ofFrontiers in microbiology · 2022Article
- Sensitive and semiquantitative detection of soil-transmitted helminth infection in stool using a recombinase polymerase amplification-based assay.PLoS neglected tropical diseases · 2021Article
- Low Sensitivity of Real Time PCRs Targeting Retrotransposon Sequences for the Detection ofPathogens (Basel, Switzerland) · 2021Article
- Schistosomiasis with a Focus on Africa.Tropical medicine and infectious disease · 2021Review
- Development of a Recombinase Polymerase Amplification Assay for Schistosomiasis Japonica Diagnosis in the Experimental Mice and Domestic Goats.Frontiers in cellular and infection microbiology · 2021Article
- Evaluation of Rapid Extraction Methods Coupled with a Recombinase Polymerase Amplification Assay for Point-of-Need Diagnosis of Post-Kala-Azar Dermal Leishmaniasis.Tropical medicine and infectious disease · 2020Article
- Recent advances in nucleic acid-based methods for detection of helminth infections and the perspective of biosensors for future development.Parasitology · 2020Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCurrent diagnostic methods for Schistosoma japonicum infection are insensitive for low-density infections. Therefore, a new diagnostic assay based on recombinase polymerase amplification (RPA) technology was established and assessed for field applification.
methodsThe S.japonicum RPA assay was developed to target highly repetitive retrotransposon SjR2 gene of S japonicum, and its sensitivity and specificity were assessed by serial dilution of S. japonicum genomic DNA and other related worm genomic DNA respectively. The RPA diagnostic validity was first evaluated in 60 fecal samples from healthy people and patients, and then compared with other diagnostic tests in 200 high-risk individuals living in endemic areas.
resultsThe real time RPA assay could detect 0.9 fg S. japonicum DNA within 15 min and distinguish S. japonicum from other worms. The validity analysis of RPA for the detection of S. japonicum in stool samples from 30 S. japonicum-infected patients and 30 healthy persons indicated 100% sensitivity and specificity. When testing 200 fecal or serum samples from a high-risk population, the percentage sensitivity of RPA was 100%, whereas that of indirect hemagglutination assay (IHA) and enzyme-linked immunosorbent assay (ELISA) were 80.3% and 85.2% respectively. In addition, the RPA presented better consistency with the stool-based tests than IHA and ELISA. Overall, the RPA was superior to other detection methods with respect to detection time, sensitivity, and convenience.
conclusionsThis is the first time we applied the RPA technology to the field evaluation of S. japonicum infection. And the results suggest that RPA-based assays can be used as a promising point-of-care test for the diagnosis of schistosomiasis.
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