ArticleInternational journal of stem cells2017
Stemness Signature of Equine Marrow-derived Mesenchymal Stem Cells.
Article in International journal of stem cells, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Multilineage Differentiation Potential of Equine Adipose-Derived Stromal/Stem Cells from Different Sources.Animals : an open access journal from MDPI · 2023Article
- Effect of Passage Number of Conditioned Medium Collected from Equine Amniotic Fluid Mesenchymal Stem Cells: Porcine Oocyte Maturation and Embryo Development.International journal of molecular sciences · 2022Article
- Regulatory effects of miR-28 on osteogenic differentiation of human bone marrow mesenchymal stem cells.Bioengineered · 2022Article
- Development of a biologically immortalized equine stem cell line.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2021Article
- The effect of equine bone marrow-derived mesenchymal stem cells on the expression of apoptotic genes in neutrophils.Veterinary medicine and science · 2021Article
- Therapeutic Use of Mesenchymal Stromal Cells: The Need for Inclusive Characterization Guidelines to Accommodate All Tissue Sources and Species.Frontiers in cell and developmental biology · 2021Review
- Coronary corium, a new source of equine mesenchymal stromal cells.Veterinary research communications · 2020Article
- Strategies of tenogenic differentiation of equine stem cells for tendon repair: current status and challenges.Stem cell research & therapy · 2019Review
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundApplication of competent cells such as mesenchymal stem cells (MSCs) for treatment of musculoskeletal disorders in equine athletes is increasingly needed. Moreover, similarities of horse and human in size, load and types of joint injuries, make horse as a good model for MSCs therapy studies. This study was designed to isolate and characterize stemness signature of equine bone marrow-derived mesenchymal stem cells (BM-MSCs).
methodsBM of three mares was aspirated and the mononuclear cells (MNCs) were isolated using density gradient. The primary MNCs were cultured and analyzed after tree passages (P3) for growth characteristics, differentiation potentials, and the expression of genes including CD29, CD34, CD44, CD90, CD105, MHC-I, MHC-II and pluripotency related genes (Nanog, Oct-4, Sox-2, SSEA-1, -3, -4) using RT-PCR or immunocytochemistry techniques.
resultsThe isolated cells in P3 were adherent and fibroblast-like in shape with doubling times of 78.15 h. Their clonogenic capacity was 8.67±4% and they were able to differentiate to osteogenic, adipogenic and chondrogenic lineages. Cells showed expression of CD29, CD44, CD90, MHC-I and Sox-2 while no expression for CD34, MHC-II, CD105, and pluripotency stemness markers was detected.
conclusionsIn conclusion, data showed that isolated cells have the basic and minimal criteria for MSCs, however, expressing only one pluripotency gene (sox-2).
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