ArticlePloS one2017
Streptococcus sanguinis induces neutrophil cell death by production of hydrogen peroxide.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 35 citations in OpenAlex.
- Trial
- A metagenomic study of the gut microbiome in patients with type 2 diabetes mellitus and myocardial infarction.Acta diabetologica · 2026Article
- Neutrophil Activation Decreases Ability to Kill Oral Streptococcus gordonii.Molecular oral microbiology · 2026Article
- Molecular mimicry revisited: novel perspectives on autoimmune disease initiation and progression.Frontiers in immunology · 2026Review
- Elucidating the Role of Neutrophils in the Inflammatory Response to Behcet Syndrome.Journal of inflammation research · 2026Review
- Article
- Microbial dysbiosis in periodontitis and peri-implantitis: pathogenesis, immune responses, and therapeutic.Frontiers in cellular and infection microbiology · 2025Review
- Symbiosis Between the Oral Microbiome and the Human Host: Microbial Homeostasis and Stability of the Host.Advances in experimental medicine and biology · 2025Review
- A novel infective endocarditis virulence factor related to multiple functions for bacterial survival in blood was discovered inbioRxiv : the preprint server for biology · 2024Article
- Insights into the Chemistry, Structure, and Biological Activity of Human Salivary MUC7 Fragments and Their Cu(II) and Zn(II) Complexes.Inorganic chemistry · 2024Article
- Neutrophil extracellular traps in bacterial infections and evasion strategies.Frontiers in immunology · 2024Review
- Oral streptococci: modulators of health and disease.Frontiers in cellular and infection microbiology · 2024Review
- Methylprednisolone pulse-enhanced neutrophil extracellular trap formation in mice with imiquimod-induced lupus-like disease, resulting in ischaemia of the femoral head cartilage.Lupus science & medicine · 2023Article
- Current Infections of the Orofacial Region: Treatment, Diagnosis, and Epidemiology.Life (Basel, Switzerland) · 2023Review
- Oral mitis group streptococci reduce infectivity of influenza A virus via acidification and H2O2 production.PloS one · 2022Article
- Diversity in Phenotypes Associated With Host Persistence and Systemic Virulence inFrontiers in microbiology · 2022Article
- Clinical, Laboratory and Radiographic Features Associated With Prolonged Hospitalization in Children With Complicated Appendicitis.Frontiers in pediatrics · 2022Article
- Post-translational modification of Streptococcus sanguinis SpxB influences protein solubility and HMolecular oral microbiology · 2021Article
- Precision targeting of bacterial pathogen via bi-functional nanozyme activated by biofilm microenvironment.Biomaterials · 2021Article
- Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus is the dominant bacterial genus in the human oral cavity and a leading cause of infective endocarditis. Streptococcus sanguinis belongs to the mitis group of streptococci and produces hydrogen peroxide (H2O2) by the action of SpxB, a pyruvate oxidase. In this study, we investigated the involvement of SpxB in survival of S. sanguinis in human blood and whether bacterial H2O2 exhibits cytotoxicity against human neutrophils. Results of a bactericidal test with human whole blood revealed that the spxB mutation in S. sanguinis is detrimental to its survival in blood. When S. sanguinis strains were exposed to isolated neutrophils, the bacterial survival rate was significantly decreased by spxB deletion. Furthermore, human neutrophils exposed to the S. sanguinis wild-type strain, in contrast to those exposed to an spxB mutant strain, underwent cell death with chromatin de-condensation and release of web-like extracellular DNA, reflecting induction of neutrophil extracellular traps (NETs). Since reactive oxygen species-mediated NET induction requires citrullination of arginine residues in histone proteins and subsequent chromatin de-condensation, we examined citrullination levels of histone in infected neutrophils. It is important to note that the citrullinated histone H3 was readily detected in neutrophils infected with the wild-type strain, as compared to infection with the spxB mutant strain. Moreover, decomposition of streptococcal H2O2 with catalase reduced NET induction. These results suggest that H2O2 produced by S. sanguinis provokes cell death of neutrophils and NET formation, thus potentially affecting bacterial survival in the bloodstream.
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