ArticleMacromolecular bioscience2017
Imaging Cell-Matrix Interactions in 3D Collagen Hydrogel Culture Systems.
Article in Macromolecular bioscience, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Hydrogel Microdroplet Based Glioblastoma Drug Screening Platform.Journal of biomedical materials research. Part A · 2026Article
- Histological Processing of Scaffolds: Challenges and Solutions.Journal of functional biomaterials · 2025Review
- Hydrogel microdroplet based glioblastoma drug screening platform.bioRxiv : the preprint server for biology · 2025Article
- Exploring New Dimensions of Tumor Heterogeneity: The Application of Single Cell Analysis to Organoid-Based 3D In Vitro Models.Advanced healthcare materials · 2023Review
- DISC-3D: dual-hydrogel system enhances optical imaging and enables correlative mass spectrometry imaging of invading multicellular tumor spheroids.Scientific reports · 2023Article
- Evaluating glioblastoma tumour sphere growth and migration in interaction with astrocytes using 3D collagen-hyaluronic acid hydrogels.Journal of materials chemistry. B · 2023Article
- Nanocomposite Hydrogels as Functional Extracellular Matrices.Gels (Basel, Switzerland) · 2023Review
- Neutrophils Culture in Collagen Gel System.Frontiers in immunology · 2022Article
- 3D hydrogel models of the neurovascular unit to investigate blood-brain barrier dysfunction.Neuronal signaling · 2021Review
- Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues.Frontiers in bioengineering and biotechnology · 2020Article
- Blended alginate/collagen hydrogels promote neurogenesis and neuronal maturation.Materials science & engineering. C, Materials for biological applications · 2019Article
- Behaviors of Glioblastoma Cells in in Vitro Microenvironments.Scientific reports · 2019Article
- Hydrogel-based scaffolds to support intrathecal stem cell transplantation as a gateway to the spinal cord: clinical needs, biomaterials, and imaging technologies.NPJ Regenerative medicine · 2018Review
- Multimodal analysis of the differential effects of cyclic strain on collagen isoform composition, fibril architecture and biomechanics of tissue engineered tendon.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
3D hydrogels better replicate in vivo conditions, and yield different results from 2D substrates. However, imaging interactions between cells and the hydrogel microenvironment is challenging because of light diffraction and poor focal depth. Here, cryosectioning and vibrating microtomy methods and fixation protocols are compared. Collagen I/III hydrogel sections (20-100 µm) are fixed with paraformaldehyde (2%-4%) and structurally evaluated. Cryosectioning damaged hydrogels, and vibrating microtomy (100 µm, 2%) yielded the best preservation of microstructure and cell integrity. These results demonstrate a potential processing method that preserves hydrogel and cell integrity, permitting imaging of cell interactions with the microenvironment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.