ArticleChembiochem : a European journal of chemical biology2017
Quantitative PCR is a Valuable Tool to Monitor the Performance of DNA-Encoded Chemical Library Selections.
Article in Chembiochem : a European journal of chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- A tailored CoA ligase-Nature catalysis · 2026Article
- Impact of library input on the hit discovery rate in DNA-encoded chemical library selections.Chemical science · 2023Article
- Recent advances in DNA-encoded dynamic libraries.RSC chemical biology · 2022Review
- Strategies for developing DNA-encoded libraries beyond binding assays.Nature chemistry · 2022Review
- Comparative evaluation of DNA-encoded chemical selections performed using DNA in single-stranded or double-stranded format.Biochemical and biophysical research communications · 2020Article
- Tumor-Targeting Peptides: The Functional Screen of Glioblastoma Homing Peptides to the Target Protein FABP3 (MDGI).Cancers · 2020Article
- DNA-Encoded Chemical Libraries: A Selection System Based on Endowing Organic Compounds with Amplifiable Information.Annual review of biochemistry · 2018Review
- Robustness of In Vitro Selection Assays of DNA-Encoded Peptidomimetic Ligands to CBX7 and CBX8.SLAS discovery : advancing life sciences R & D · 2018Article
- DNA-encoded chemical libraries - achievements and remaining challenges.FEBS letters · 2018Review
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Authors and funding
4 authors.
Funding
Abstract
Phage-display libraries and DNA-encoded chemical libraries (DECLs) represent useful tools for the isolation of specific binding molecules from large combinatorial sets of compounds. With both methods, specific binders are recovered at the end of affinity capture procedures by using target proteins of interest immobilized on a solid support. However, although the efficiency of phage-display selections is routinely quantified by counting the phage titer before and after the affinity capture step, no similar quantification procedures have been reported for the characterization of DECL selections. In this article, we describe the potential and limitations of quantitative PCR (qPCR) methods for the evaluation of selection efficiency by using a combinatorial chemical library with more than 35 million compounds. In the experimental conditions chosen for the selections, a quantification of DNA input/recovery over five orders of magnitude could be performed, revealing a successful enrichment of abundant binders, which could be confirmed by DNA sequencing. qPCR provided rapid information about the performance of selections, thus facilitating the optimization of experimental conditions.
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