ArticleArchives of virology2017
Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR.
Article in Archives of virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.
- Viral Co-Infection in Bats: A Systematic Review.Viruses · 2023Pooled it
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- Co-infections and Reactivation of some Herpesviruses (HHV) and Measles Virus (MeV) in Egyptian Cancer Patients infected with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).Journal of the Egyptian National Cancer Institute · 2025Observational
- Unraveling metabolic signatures in SARS-CoV-2 variant infections using multiomics analysis.Frontiers in immunology · 2024Article
- Nanotechnology-based diagnostic methods for coronavirus: From nucleic acid extraction to amplification.Biosensors & bioelectronics: X · 2023Review
- Experimental Infection and Transmission of SARS-CoV-2 Delta and Omicron Variants among Beagle Dogs.Emerging infectious diseases · 2023Article
- Susceptibility to SARS-CoV-2 and MERS-CoV in Beagle Dogs.Animals : an open access journal from MDPI · 2023Article
- A Retrospective Population Study of 385 191 Positive Real-Time Reverse Transcription-Polymerase Chain Reaction Tests For SARS-CoV-2 from a Single Laboratory in Katowice, Poland from April 2020 to July 2022.Medical science monitor : international medical journal of experimental and clinical research · 2023Article
- CRISPR-based assays for rapid detection of SARS-CoV-2.Methods (San Diego, Calif.) · 2022Review
- Acquired insights from the long-term surveillance of SARS-CoV-2 RNA for COVID-19 monitoring: The case of Monterrey Metropolitan Area (Mexico).Environmental research · 2022Article
- Surface plasmon resonance biosensor with laser heterodyne feedback for highly-sensitive and rapid detection of COVID-19 spike antigen.Biosensors & bioelectronics · 2022Article
- Article
- Diagnostic performance and clinical feasibility of a novel one-step RT-qPCR assay for simultaneous detection of multiple severe acute respiratory syndrome coronaviruses.Archives of virology · 2022Article
- Highly sensitive, scalable, and rapid SARS-CoV-2 biosensor based on InNano research · 2022Article
- Analytical performances of different diagnostic methods for SARS-CoV-2 virus - A review.Sensors international · 2022Review
- COVID-19 surveillance in wastewater: An epidemiological tool for the monitoring of SARS-CoV-2.Frontiers in cellular and infection microbiology · 2022Review
- Human Body Performance with COVID-19 Affectation According to Virus Specification Based on Biosensor Techniques.Sensors (Basel, Switzerland) · 2021Review
- Recent advances in detection technologies for COVID-19.Talanta · 2021Review
- COVID-19 rhapsody: Rage towards advanced diagnostics and therapeutic strategy.Journal of pharmaceutical analysis · 2021Review
- Electrochemical investigations for COVID-19 detection-A comparison with other viral detection methods.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, and transmission, methods for a rapid and accurate differential diagnosis are important. Therefore, the aim of this study was to develop a duplex real-time reverse transcription (RT)-PCR method for the simultaneous detection of these viruses. Primers and probes that target the conserved spike S2 region of human SARS-CoV, MERS-CoV, and their related bat CoVs were designed. The results of real-time RT-PCR showed specific reactions for each virus with adequate detection limits of 50-100 copies/mL and 5-100 copies/mL using pUC57-SARS-pS2 (a template for SARS-CoV) and pGEM-MERS-S2 (a template for MERS-CoV), respectively. In addition, this real-time RT-PCR system was able to detect the target viruses SARS-like bat CoV and MERS-CoV in bat fecal samples and sputum of MERS patients, respectively. Therefore, this newly developed real-time RT-PCR method is expected to detect not only SARS-CoV and MERS-CoV in humans but also several bat CoVs that are closely related to these viruses in bats.
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