ArticleThe Biochemical journal1987
Purification, characterization and cellular localization of 5'-nucleotidase from Torpedo electric organ.
Article in The Biochemical journal, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 49 citations in OpenAlex.
- Synthesis, Characterization, andACS medicinal chemistry letters · 2020Article
- A Photo-clickable ATP-Mimetic Reveals Nucleotide Interactors in the Membrane Proteome.Cell chemical biology · 2020Article
- Cellular function and molecular structure of ecto-nucleotidases.Purinergic signalling · 2012Review
- Ecto-5'-nucleotidase: Structure function relationships.Purinergic signalling · 2006Article
- Regulation of the ecto-nucleotidase pathway in rat hippocampal nerve terminals.Neurochemical research · 2001Article
- Ecto-5'-nucleotidase from a human colon adenocarcinoma cell line. Correlation between enzyme activity and levels in intact cells.Molecular and cellular biochemistry · 1998Article
- Action of suramin upon ecto-apyrase activity and synaptic depression of Torpedo electric organ.British journal of pharmacology · 1996Article
- Isolation and characterization of the ecto-5'-nucleotidase from a rat glioblastoma cell line.Molecular and cellular biochemistry · 1992Article
- 5'-Nucleotidase: molecular structure and functional aspects.The Biochemical journal · 1992Review
- Soluble low-Km 5'-nucleotidase from electric-ray (Torpedo marmorata) electric organ and bovine cerebral cortex is derived from the glycosyl-phosphatidylinositol-anchored ectoenzyme by phospholipase C cleavage.The Biochemical journal · 1992Article
- Association of the HNK-1 epitope with 5'-nucleotidase from Torpedo marmorata (electric ray) electric organ.The Biochemical journal · 1991Article
- Emerging functional roles for the glycosyl-phosphatidylinositol membrane protein anchor.The Journal of membrane biology · 1990Review
- Membrane proteins of synaptic vesicles and cytoskeletal specializations at the node of Ranvier in electric ray and rat.Cell and tissue research · 1989Article
- 5'-Nucleotidases of chicken gizzard and human pancreatic adenocarcinoma cells are anchored to the plasma membrane via a phosphatidylinositol-glycan.The Biochemical journal · 1989Article
- [Cholinergic nerve endings: cellular function and molecular structure].Die Naturwissenschaften · 1987Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
5'-Nucleotidase was isolated from the electric organ of the electric ray Torpedo marmorata after solubilization in Triton X-100 and deoxycholate by affinity chromatography on concanavalin A-Sepharose and AMP-Sepharose. The purified enzyme has a Km for AMP of 38 microM, with a maximal velocity of 31 units/mg of protein. Of the purine and pyrimidine mononucleotides, AMP is hydrolysed most effectively. beta-Glycerophosphate, phosphoenolpyruvate and p-nitrophenyl phosphate are not substrates for the enzyme. Adenosine 5'-[alpha, beta-methylene]diphosphate, ADP and ATP are competitive inhibitors in this order of potency. Concanavalin A inhibits enzyme activity in a non-competitive manner. Whereas Mg2+, Ca2+ and Sr2+ activate enzyme activity in the millimolar range, Hg2+, and in particular Pb2+ and Zn2+, inhibit enzyme activity. On SDS/polyacrylamide-gel electrophoresis the enzyme has an apparent Mr of 62000, whereas that of the native deoxycholate-enzyme complex is 131000. An antiserum raised against the native enzyme inhibits enzyme activity. Inhibition studies suggest the presence of tissue-specific variants of the enzyme. By immunohistochemical analysis the enzyme can be localized to the ramifications of nerve terminals in the electric organ.
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