ArticleMicrobial cell factories2017
High-level extracellular protein production in Bacillus subtilis using an optimized dual-promoter expression system.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 127 citations in OpenAlex.
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- Synergistic strategies for high production of Geobacillus stearothermophilus α-amylase in Bacillus subtilis.Journal of industrial microbiology & biotechnology · 2026Article
- Establishment of the CRISPR-Cpf1 gene editing system inSynthetic and systems biotechnology · 2025Article
- Enhancing High-Level Food-Grade Expression of Glutamate Decarboxylase and Its Application in the Production of γ-Aminobutyric Acid.Journal of microbiology and biotechnology · 2024Article
- Genetic identification and expression optimization of a novel protease HapR fromFrontiers in bioengineering and biotechnology · 2024Article
- Induction of the CtsR regulon improves Xylanase production in Bacillus subtilis.Microbial cell factories · 2023Article
- Efficient production of α-monoglucosyl hesperidin by cyclodextrin glucanotransferase from Bacillus subtilis.Applied microbiology and biotechnology · 2023Article
- Enhanced extracellular production of raw starch-degrading α-amylase in Bacillus subtilis through expression regulatory element modification and fermentation optimization.Microbial cell factories · 2023Article
- Pullulanase: unleashing the power of enzyme with a promising future in the food industry.Frontiers in bioengineering and biotechnology · 2023Review
- The Superiority ofMicroorganisms · 2022Article
- Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis.Microbial cell factories · 2022Article
- Enhanced Extracellular Production and Characterization of Sucrose Isomerase inFoods (Basel, Switzerland) · 2022Article
- Enhanced extracellular raw starch-degrading α-amylase production in Bacillus subtilis by promoter engineering and translation initiation efficiency optimization.Microbial cell factories · 2022Article
- Cis-Element Engineering Promotes the Expression ofInternational journal of molecular sciences · 2022Article
- Secretory production of an engineered cutinase in Bacillus subtilis for efficient biocatalytic depolymerization of polyethylene terephthalate.Bioprocess and biosystems engineering · 2022Article
- An operator-based expression toolkit forProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Optimal Secretory Expression of Acetaldehyde Dehydrogenase fromMolecules (Basel, Switzerland) · 2022Article
- Heterologous expression of 4α-glucanotransferase: overproduction and properties for industrial applications.World journal of microbiology & biotechnology · 2022Review
- Egr1/HSP70 Promoter-Driven Activation of Gene Expression for Synergistic Anti-Hepatoma Using PEI-MZF Nanoparticles and Radiation.International journal of nanomedicine · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe recently constructed a Bacillus subtilis strain (CCTCC M 2016536) from which we had deleted the srfC, spoIIAC, nprE, aprE and amyE genes. This strain is capable of robust recombinant protein production and amenable to high-cell-density fermentation. Because the promoter is among the factors that influence the production of target proteins, optimization of the initial promoter, P
resultsUsing the enzyme β-cyclodextrin glycosyltransferase (β-CGTase) as a reporter protein and B. subtilis CCTCC M 2016536 as the host, nine plasmids equipped with single promoters were screened using shake-flask cultivation. The plasmid containing the P
conclusionsThe dual-promoter P
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.