ArticleScientific reports2017
CRISPR knockout rat cytochrome P450 3A1/2 model for advancing drug metabolism and pharmacokinetics research.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 54 citations in OpenAlex.
- Effects of mallotus furetianus extract on CYP3A activities in rats.Scientific reports · 2025Article
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- The role of CYP1A1/2 in cholesterol ester accumulation provides a new perspective for the treatment of hypercholesterolemia.Acta pharmaceutica Sinica. B · 2023Article
- Cytochrome P450s in algae: Bioactive natural product biosynthesis and light-driven bioproduction.Acta pharmaceutica Sinica. B · 2022Review
- Recent Advances in the Production of Genome-Edited Rats.International journal of molecular sciences · 2022Review
- CRISPR-Cas9: A method for establishing rat models of drug metabolism and pharmacokinetics.Acta pharmaceutica Sinica. B · 2021Review
- Rat models of human diseases and related phenotypes: a systematic inventory of the causative genes.Journal of biomedical science · 2020Review
- Generation of novel genetically modified rats to reveal the molecular mechanisms of vitamin D actions.Scientific reports · 2020Article
- Effect of You-Gui Yin on the Activities of Seven Cytochrome P450 Isozymes in Rats.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- Using CRISPR/Cas9 to model human liver disease.JHEP reports : innovation in hepatology · 2019Review
- Current trends in drug metabolism and pharmacokinetics.Acta pharmaceutica Sinica. B · 2019Review
- Role of vitamin D receptor in the regulation ofActa pharmaceutica Sinica. B · 2019Review
- Humanized UGT2 and CYP3A transchromosomic rats for improved prediction of human drug metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- CRISPR/Cas9, the Powerful New Genome-Editing Tool for Putative Therapeutics in Obesity.Journal of molecular neuroscience : MN · 2018Review
- CRISPR/Cas9 Genetic Modification ofDrug metabolism and disposition: the biological fate of chemicals · 2017Article
- Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytochrome P450 (CYP) 3A accounts for nearly 30% of the total CYP enzymes in the human liver and participates in the metabolism of over 50% of clinical drugs. Moreover, CYP3A plays an important role in chemical metabolism, toxicity, and carcinogenicity. New animal models are needed to investigate CYP3A functions, especially for drug metabolism. In this report, Cyp3a1/2 double knockout (KO) rats were generated by CRISPR-Cas9 technology, and then were characterized for viability and physiological status. The Cyp3a1/2 double KO rats were viable and fertile, and had no obvious physiological abnormities. Compared with the wild-type (WT) rat, Cyp3a1/2 expression was completely absent in the liver of the KO rat. In vitro and in vivo metabolic studies of the CYP3A1/2 substrates indicated that CYP3A1/2 was functionally inactive in double KO rats. The Cyp3a1/2 double KO rat model was successfully generated and characterized. The Cyp3a1/2 KO rats are a novel rodent animal model that will be a powerful tool for the study of the physiological and pharmacological roles of CYP3A, especially in drug and chemical metabolism in vivo.
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