ArticleScientific reports2017
Rapid and sensitive diagnoses of dry root rot pathogen of chickpea (Rhizoctonia bataticola (Taub.) Butler) using loop-mediated isothermal amplification assay.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- Development of a multipurpose diagnostic marker using PCR, real-time PCR, and LAMP assays for rapid detection ofFrontiers in plant science · 2026Article
- A comprehensive guide to loop-mediated isothermal amplification, an emerging diagnostic tool for plant pathogenic fungi.Frontiers in plant science · 2025Review
- LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives.Life (Basel, Switzerland) · 2024Review
- Reverse transcription loop-mediated isothermal amplification (RT-LAMP) primer design based on Indonesia SARS-CoV-2 RNA sequence.Journal, genetic engineering & biotechnology · 2023Article
- Prevalence of groundnut dry root rot (Frontiers in fungal biology · 2023Article
- A loop-mediated isothermal amplification (LAMP) assay for the detection of Cryptotermes brevis West Indian drywood termite (Blattodea: Kalotermitidae).Scientific reports · 2022Article
- First Report of Crown Gall of Kiwifruit (International journal of molecular sciences · 2021Article
- Detection and Control ofBiology · 2021Article
- A sick plot-based protocol for dry root rot disease assessment in field-grown chickpea plants.Applications in plant sciences · 2021Article
- Temperature and Soil Moisture Stress Modulate the Host Defense Response in Chickpea During Dry Root Rot Incidence.Frontiers in plant science · 2021Article
- A rapid colorimetric LAMP assay for detection of Rhizoctonia solani AG-1 IA causing sheath blight of rice.Scientific reports · 2020Article
- Rapid detection of Puccinia triticina causing leaf rust of wheat by PCR and loop mediated isothermal amplification.PloS one · 2018Article
- Soil fungal biodiversity and pathogen identification of rotten disease in Aconitum carmichaelii (Fuzi) roots.PloS one · 2018Article
- Exploring Combined Effect of Abiotic (Soil Moisture) and Biotic (Frontiers in plant science · 2018Article
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3 authors.
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Abstract
Dry root rot (DRR) caused by the fungus Rhizoctonia bataticola (Taub.) Butler, is an emerging disease in chickpea. The disease is often mistaken with other root rots like Fusarium wilt, collar rot and black root rot in chickpea. Therefore, its timely and specific detection is important. Current detection protocols are either based on mycological methods or on protocols involving DNA amplification by polymerase chain reaction (PCR). Here we report the rapid and specific detection of R. bataticola using loop-mediated isothermal amplification (LAMP) assay targeting fungal specific 5.8S rDNA sequence for visual detection of R. bataticola. The reaction was optimized at 63 °C for 75 min using minimum 10 fg of DNA. After adding SYBR Green I in LAMP products, the amplification was found to be highly specific in all the 94 isolates of R. bataticola collected from diverse geographical regions as well as DRR infected plants and sick soil. No reaction was found in other pathogenic fungi infecting chickpea (Fusarium oxysporum f. sp. ciceris, Rhizoctonia solani, Sclerotium rolfsii and Fusarium solani) and pigeonpea (Fusarium udum and Phytophthora cajani). The standardised LAMP assay with its simplicity, rapidity and specificity is very useful for the visual detection of this emerging disease in chickpea.
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