ArticlePharmacognosy magazine
High Performance Liquid Chromatography-Diode Array Detector Method for the Simultaneous Determination of Five Compounds in the Pulp and Seed of Sea Buckthorn.
Article in Pharmacognosy magazine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 16 citations in OpenAlex.
- Sea buckthorn polyphenols improve lipid metabolism via gut-liver axis modulation in db/db mice.Food & nutrition research · 2026Article
- Growth and physiological response of YuluPlant signaling & behavior · 2024Article
- Anti-Platelet Activity of Sea Buckthorn Seeds and Its Relationship with Thermal Processing.Foods (Basel, Switzerland) · 2024Article
- Unraveling the Antioxidant Activity ofInternational journal of molecular sciences · 2023Article
- Flavonoid Extract from Seed Residues ofAntioxidants (Basel, Switzerland) · 2023Article
- Article
- Effect of Sea Buckthorn on Plasma Glucose in Individuals with Impaired Glucose Regulation: A Two-Stage Randomized Crossover Intervention Study.Foods (Basel, Switzerland) · 2021Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
contextSea buckthorn (
objectiveA reversed-phase rapid-resolution liquid-chromatography method with diode array detector was developed for simultaneous determination of protocatechuic acid, rutin, quercetin, kaempferol, and isorhamnetin in the pulp and seed of sea buckthorn, a widely used traditional Chinese medicine for promoting metabolism and treating scurvy and other diseases. SETTINGS AND
designCompounds were separated on an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm; USA) with gradient elution using methanol and 0.4% phosphoric acid (v/v) at 1.0 mL/min. Detection wavelength was set at 280 nm. MATERIALS AND
methodsThe fruits of wild sea buckthorn were collected from Wushi County in Aksu, Xinjiang Province. STATISTICAL PERFORMANCES: The RSD of precision test of the five compounds were in the range of 0.60-2.22%, and the average recoveries ranged from 97.36% to 101.19%. Good linearity between specific chromatographic peak and component qualities were observed in the investigated ranges for all the analytes (
resultsThe proposed method was successfully applied to determine the levels of five active components in sea buckthorn samples from Aksu in Xinjiang.
conclusionsThe proposed method is simple, fast, sensitive, accurate, and suitable for quantitative assessment of the pulp and seed of sea buckthorn. SUMMARY: Quantitative analysis method of protocatechuic acid, rutin, quercetin, kaempferol, and isorhamnetin in the extract of sea buckthorn pulp and seed is developed by high-performance liquid chromatography (HPLC) diode array detection.This method is simple and accurate; has strong specificity, good precision, and high recovery rate; and provides a reliable basis for further development of the substances in the pulp and seed of sea buckthorn.The method is widely used for content determination of active ingredients or physiologically active components in traditional Chinese medicine and its preparation
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