ArticleInternational journal of stem cells2017
Efficacy of Human Platelet Rich Fibrin Exudate vs Fetal Bovine Serum on Proliferation and Differentiation of Dental Pulp Stem Cells.
Article in International journal of stem cells, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies.Clinical oral investigations · 2020Pooled it
- Enhancing the Odontogenic Potential of Human Dental Pulp Stem Cells via Platelet-Rich Plasma Exosomes through Modulation of TGF-β1 and Dentin Sialophosphoprotein.European journal of dentistry · 2026Article
- In Vitro Evaluation of the Regenerative Potential of Autologous Platelet-Rich Fibrin (PRF) on Human Primary Periodontal Ligament Cells.International journal of molecular sciences · 2025Article
- Impact of dental pulp cells-derived small extracellular vesicles on the properties and behavior of dental pulp cells: an in-vitro study.BMC oral health · 2025Article
- Human dental pulp stem/stromal cells in clinical practice.World journal of stem cells · 2024Article
- Research progress on optimization ofFrontiers in bioengineering and biotechnology · 2024Review
- Comparative Evaluation of the Effect of Two Platelet Concentrates (a-PRF and L-PRF) on the Cellular Activity of Pre-osteoblastic MG-63 Cell Line: An in vitro Study.Journal of dentistry (Shiraz, Iran) · 2023Article
- Changes in Migratory Speed Rate of Human Dental Pulp Stromal Cells Cultured in Advanced Platelet-Rich Fibrin.European journal of dentistry · 2023Article
- Analysis of Thrombin-Activated Platelet-Derived Exosome (T-aPDE) Potential for Dental Pulp Regeneration: In-Vitro Study.European journal of dentistry · 2023Article
- Comparison of in vitro biocompatibility and antibacterial activity of two calcium silicate-based materials.Journal of materials science. Materials in medicine · 2021Article
- The distribution pattern and growth factor level in platelet-rich fibrin incorporated skin-derived mesenchymal stem cells: AnVeterinary world · 2020Article
- Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells.Experimental and therapeutic medicine · 2020Article
- Early therapeutic effect of platelet-rich fibrin combined with allogeneic bone marrow-derived stem cells on rats' critical-sized mandibular defects.World journal of stem cells · 2020Article
- Injectable platelet-rich fibrin influences the behavior of gingival mesenchymal stem cells.Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie · 2020Article
- Xeno-free trans-differentiation of adipose tissue-derived mesenchymal stem cells into glial and neuronal cells.American journal of stem cells · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivesThe imperative role of dental pulp stem cells (DPSCs) in regenerative therapy demands an
methodsThe direct method was used to prepare hPRF exudate. hDPSCs were isolated from impacted mandibular third molars of twelve donors by the outgrowth method. For cell viability and proliferation rate testing, 96 well plates were used and the assay was done in duplicate and the trial repeated four times under the same conditions. Six wells were used to contain 10% FBS, serum free media, 1%, 5%, 10% and 20% concentrations of hPRF exudates, respectively. The proliferation assay was carried out by MTS tetrazolium cell proliferation assay kit and Elisa reader. The study design for osteodifferentiation protocol was exactly as the proliferation one and instead the assay was carried out by alizarin red with Elisa reader.
resultsCompared to 10% FBS, 10% hPRF exudate was the optimum concentration for hDPSCs proliferation, while 1% hPRF exudate was the optimum concentration for osteodifferentiation of hDPSCs. Conclusions: Avoiding the risk of zoonosis which may be occurred with FBS, it is recommended to use 10% hPRF exudate for proliferation and 1% for osteodifferentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.