ArticleMicrobial ecology2017
Attached and Suspended Denitrifier Communities in Pristine Limestone Aquifers Harbor High Fractions of Potential Autotrophs Oxidizing Reduced Iron and Sulfur Compounds.
Article in Microbial ecology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- Pooled it
- Microbial ecology of shallow groundwater ecosystems.Nature reviews. Microbiology · 2026Review
- Two worlds beneath: Distinct microbial strategies of the rock-attached and planktonic subsurface biosphere.Microbiome · 2026Article
- Exploring microbial players for metagenomic profiling of carbon cycling bacteria in sundarban mangrove soils.Scientific reports · 2025Article
- Insights into the microbiome of mine drainage from the Mária mine in Rožňava, Slovakia: a metagenomic approach.Frontiers in microbiology · 2025Article
- Primary succession of microbial communities in an aquifer from the Covey Hill formation in Quebec, Canada.Frontiers in microbiology · 2025Article
- Effects of error, chimera, bias, and GC content on the accuracy of amplicon sequencing.mSystems · 2023Article
- Microbial degradation and assimilation of veratric acid in oxic and anoxic groundwaters.Frontiers in microbiology · 2023Article
- Biological Oxidation of Fe(II)-Bearing Smectite by Microaerophilic Iron OxidizerEnvironmental science & technology · 2022Article
- Unraveling Fe(II)-Oxidizing Mechanisms in a Facultative Fe(II) Oxidizer, Sideroxydans lithotrophicus Strain ES-1, via Culturing, Transcriptomics, and Reverse Transcription-Quantitative PCR.Applied and environmental microbiology · 2022Article
- The microbial dimension of submarine groundwater discharge: current challenges and future directions.FEMS microbiology reviews · 2021Review
- Nitrate Removal by a Novel Lithoautotrophic Nitrate-Reducing, Iron(II)-Oxidizing Culture Enriched from a Pyrite-Rich Limestone Aquifer.Applied and environmental microbiology · 2021Article
- Distinct bacterial communities in tropical island aquifers.PloS one · 2020Article
- Article
- Spring Water of an Alpine Karst Aquifer Is Dominated by a Taxonomically Stable but Discharge-Responsive Bacterial Community.Frontiers in microbiology · 2019Article
- Genomes of Neutrophilic Sulfur-Oxidizing Chemolithoautotrophs Representing 9 Proteobacterial Species From 8 Genera.Frontiers in microbiology · 2019Article
- High Microbial Diversity Despite Extremely Low Biomass in a Deep Karst Aquifer.Frontiers in microbiology · 2018Article
- Variation of microbial communities and functional genes during the biofilm formation in raw water distribution systems and associated effects on the transformation of nitrogen pollutants.Environmental science and pollution research international · 2017Article
- Nitrogen Loss from Pristine Carbonate-Rock Aquifers of the Hainich Critical Zone Exploratory (Germany) Is Primarily Driven by Chemolithoautotrophic Anammox Processes.Frontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxygen and nitrate availability as well as the presence of suitable organic or inorganic electron donors are strong drivers of denitrification; however, the factors influencing denitrifier abundance and community composition in pristine aquifers are not well understood. We explored the denitrifier community structure of suspended and attached groundwater microorganisms in two superimposed limestone aquifer assemblages with contrasting oxygen regime in the Hainich Critical Zone Exploratory (Germany). Attached communities were retrieved from freshly crushed parent rock material which had been exposed for colonization in two groundwater wells (12.7 and 48 m depth). Quantitative PCR and amplicon pyrosequencing of nirK and nirS genes encoding copper-containing or cytochrome cd1 heme-type nitrite reductase, respectively, and of bacterial 16S ribosomal RNA genes showed a numerical predominance of nirS-type denitrifiers in both attached and suspended groundwater communities and a dominance of nirS-type denitrifiers closely related to the autotrophic thiosulfate- and hydrogen-oxidizing Sulfuritalea hydrogenivorans and the iron- and sulfide-oxidizing Sideroxydans lithotrophicus ES-1. Potential rates of nitrate reduction in association with exposed crushed rock material were higher with an inorganic electron donor (thiosulfate) compared to an organic electron donor (fumarate/acetate) in the upper aquifer assemblage but similar in the lower, oxic aquifer. Our results have clearly demonstrated that groundwater from pristine limestone aquifers harbors diverse denitrifier communities which appear to selectively attach to rock surfaces and harbor a high potential for nitrate reduction. Our findings suggest that the availability of suitable inorganic versus organic electron donors rather than oxygen availability shapes denitrifier communities and their potential activity in these limestone aquifers.
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