ArticleBMC cell biology2017
Bmp-12 activates tenogenic pathway in human adipose stem cells and affects their immunomodulatory and secretory properties.
Article in BMC cell biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- Grafts in tendon repair.Materials today. Bio · 2026Review
- Comprehensive analysis of GDFs as therapeutic targets and prognosis biomarkers in gastric cancer.Medicine · 2025Article
- Biological Augmentation Using Electrospun Constructs with Dual Growth Factor Release for Rotator Cuff Repair.ACS applied bio materials · 2025Article
- Tenogenic Cues Are Biochemically and Environmentally Distinct for Tendon Stem Cells and Mesenchymal/Stromal Stem Cells.Stem cells international · 2025Article
- Article
- Mechanobiological Strategies to Enhance Ovine (Animals : an open access journal from MDPI · 2024Article
- Recent Advances in the Use of Stem Cells in Tissue Engineering and Adjunct Therapies for Tendon Reconstruction and Future Perspectives.International journal of molecular sciences · 2024Review
- Mesoporous Particle Embedded Nanofibrous Scaffolds Sustain Biological Factors for Tendon Tissue Engineering.ACS materials Au · 2023Article
- Dexamethasone Is Not Sufficient to Facilitate Tenogenic Differentiation of Dermal Fibroblasts in a 3D Organoid Model.Biomedicines · 2023Article
- Stem Cell Applications and Tenogenic Differentiation Strategies for Tendon Repair.Stem cells international · 2023Review
- Age-related cellular and microstructural changes in the rotator cuff enthesis.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Role of Yes-associated protein (YAP) in regulation of mesenchymal stem cell tenogenic differentiation.Journal of molecular histology · 2022Article
- Mesenchymal Stem Cell Sheets for Engineering of the Tendon-Bone Interface.Tissue engineering. Part A · 2022Article
- Review
- The Effect of L-Ascorbic Acid and Serum Reduction on Tenogenic Differentiation of Human Mesenchymal Stromal Cells.International journal of stem cells · 2021Article
- Tendon Tissue Repair in Prospective of Drug Delivery, Regenerative Medicines, and Innovative Bioscaffolds.Stem cells international · 2021Review
- Comparative Analysis of Tenogenic Gene Expression in Tenocyte-Derived Induced Pluripotent Stem Cells and Bone Marrow-Derived Mesenchymal Stem Cells in Response to Biochemical and Biomechanical Stimuli.Stem cells international · 2021Article
- Article
- In Vitro Innovation of Tendon Tissue Engineering Strategies.International journal of molecular sciences · 2020Review
- Copper Does Not Induce Tenogenic Differentiation but Promotes Migration and Increases Lysyl Oxidase Activity in Adipose-Derived Mesenchymal Stromal Cells.Stem cells international · 2020Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCell-based therapy is a treatment method in tendon injuries. Bone morphogenic protein 12 (BMP-12) possesses tenogenic activity and was proposed as a differentiating factor for stem cells directed to transplantation. However, BMPs belong to pleiotropic TGF-β superfamily and have diverse effect on cells. Therefore, the aim of this study was to determine if BMP-12 induces tenogenic differentiation of human adipose stem cells (hASCs) and how it affects other features of this population.
resultsHuman ASCs from 6 healthy donors were treated or not with BMP-12 (50 or 100 ng/ml, 7 days) and tested for gene expression (COLL1, SCX, MKH, DCN, TNC, RUNX2), protein expression (COLL1, COLL3, MKH), proliferation, migration, secretory activity, immunomodulatory properties and susceptibility to oxidative stress. RT-PCR revealed up-regulation of SCX, MKH and RUNX2 genes in BMP-12 treated cells (2.05, 2.65 and 1.87 fold in comparison to control, respectively, p < 0.05) and Western Blot revealed significant increase of COLL1 and MHK expression after BMP-12 treatment. Addition of BMP-12 significantly enhanced secretion of VEGF, IL-6, MMP-1 and MPP-8 by hASCs while had no effect on TGF-β, IL-10, EGF and MMP-13. Moreover, BMP-12 presence in medium attenuated inhibitory effect of hASCs on allo-activated lymphocytes proliferation. At the same time BMP-12 displayed no influence on hASCs proliferation, migration and susceptibility to oxidative stress.
conclusionBMP-12 activates tenogenic pathway in hASCs but also affects secretory activity and impairs immunomodulatory potential of this population that can influence the clinical outcome after cell transplantation.
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Registered trials
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