ArticleJournal of negative results in biomedicine2017
Antimicrobial activity of HL-60 cells compared to primary blood-derived neutrophils against Staphylococcus aureus.
Article in Journal of negative results in biomedicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- Human neutrophilic cells express anti-inflammatory EBI3 in response to Neisseria meningitidis.Journal of neuroimmunology · 2026Article
- IMDM-20 enhances neutrophilic features during 1.3 % DMSO-mediated differentiation of HL-60 cells.Biochemistry and biophysics reports · 2025Article
- A bipartite bacterial virulence factor targets the complement system and neutrophil activation.The EMBO journal · 2025Article
- HL-60 cells as a valuable model to study LPS-induced neutrophil extracellular traps release.Heliyon · 2024Article
- Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium subspecies paratuberculosis in vitro.Veterinary research · 2023Article
- Article
- Isolation methods determine human neutrophil responses after stimulation.Frontiers in immunology · 2023Article
- NETome: A model to Decode the Human Genome and Proteome of Neutrophil Extracellular Traps.Scientific data · 2022Article
- [The characteristics of neutrophil extracellular traps produced by all-trans retinoic acid-induced dHL-60 under PMA stimulation].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2022Article
- Modulatory Contribution of Oxygenating Hydrogels and Polyhexamethylene Biguanide on the Antimicrobial Potency of Neutrophil-like Cells.ACS biomaterials science & engineering · 2022Article
- Responses of promyelocytic leukemia HL60 cells as an inflammatory cell lineage model to silica microparticles used to coat blood collection tubes.International journal of implant dentistry · 2022Article
- Sourcing cells forFrontiers in medical technology · 2022Review
- Comparison of Gene Editing versus a Neutrophil Elastase Inhibitor as Potential Therapies forJournal of cellular immunology · 2022Article
- Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics.Scientific reports · 2021Article
- Differentiation of HL-60 cells in serum-free hematopoietic cell media enhances the production of neutrophil extracellular traps.Experimental and therapeutic medicine · 2021Article
- Immunomodulation: Immunoglobulin Preparations Suppress Hyperinflammation in a COVID-19 ModelFrontiers in immunology · 2021Article
- For Better or for Worse: A Look Into Neutrophils in Traumatic Spinal Cord Injury.Frontiers in cellular neuroscience · 2021Review
- Studying Neutrophil Function in vitro: Cell Models and Environmental Factors.Journal of inflammation research · 2021Review
- Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention ofMicroorganisms · 2020Article
- What Is the Evolutionary Fingerprint in Neutrophil Granulocytes?International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe human leukemia cell line HL-60 is considered an alternative cell culture model to study neutrophil differentiation and migration. The aim of this study was to characterize the suitability of HL-60 cells differentiated to neutrophil-like cells (nHL-60) as substitute for blood-derived human neutrophils to investigate the interaction of neutrophils with Staphylococcus aureus.
methodsFor this purpose, antimicrobial activity, bacterial uptake, production of reactive oxygen species and the release of neutrophil extracellular traps (NETs) by nHL-60 cells were analyzed and compared to primary blood-derived neutrophils using Staphylococcus aureus as important human and animal pathogen.
resultsOverall, the antimicrobial activities of nHL-60 cells were distinctly lower compared to blood-derived neutrophils. Furthermore, production of reactive oxygen species as well as NET formation was clearly impaired in nHL-60 cells.
conclusionThis study indicates that HL-60 cells are of limited usage as an alternative model to study antimicrobial functions of neutrophils against Staphylococcus aureus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.