Evidence map›Paper›PMID 28214223›Full record

ArticleDiagnostic microbiology and infectious disease2017

Evaluation of a field test for antibodies against Chlamydia trachomatis during trachoma surveillance in Nepal.

Michelle J Sun, Andrea I Zambrano, Laura Dize, Beatriz Munoz, Sarah Gwyn, Sailesh Mishra, Diana L Martin, Shekhar Sharma, Sheila K West

Abstract readComparative StudyEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostic microbiology and infectious disease, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Trial
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Michelle J SunDana Center for Preventive Ophthalmology, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD.
Andrea I ZambranoDana Center for Preventive Ophthalmology, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD.
Laura DizeInternational Chlamydia Laboratory, Johns Hopkins University Baltimore, MD, USA.
Beatriz MunozDana Center for Preventive Ophthalmology, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD.
Sarah GwynIHRC, Inc. Contractor at the Centers for Disease Control and Prevention, Atlanta, GA, USA.
Sailesh MishraNepal Netra Jyoti Sangh, Kathmandu, Nepal.
Diana L MartinDivision of Parasitic Diseases and Malaria, Center for Disease Control and Prevention, Atlanta, GA, USA.
Shekhar SharmaNepal Netra Jyoti Sangh, Kathmandu, Nepal.
Sheila K WestDana Center for Preventive Ophthalmology, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD. Electronic address: shwest@jhmi.edu.

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

purposeTesting for antibodies to Chlamydia trachomatis has potential as a surveillance tool. Our evaluation compares lateral flow assays (LFAs) during surveillance surveys in Nepal with Multiplex bead array (MBA). Fifty children were randomly sampled from each of 15 random clusters in two districts of Nepal. Finger prick blood samples were collected from 1509 children and tested onsite for anti-Pgp3 antibodies by LFA. The LFA was read at 30min as negative, positive, or invalid. Tests results were also rated as difficult to read ("equivocal"). Blood was processed at Johns Hopkins University using the MBA.

resultsThe LFA had agreement of 40.0% for MBA-positive samples and 99.3% for MBA-negative samples. Inter-reader reliability was kappa=0.65 (95% CI=0.56-0.74). If the equivocal results (7%) could be decreased, reliability could be improved.

conclusionsFurther optimization and testing of the LFA test are needed to improve agreement with MBA and the interpretation of the results.

Indexed as

Antibodies, BacterialAntigens, BacterialBacterial ProteinsChildChild, PreschoolChlamydia trachomatisChromatography, AffinityEpidemiological MonitoringFemaleHumansImmunomagnetic SeparationInfantMaleNepalSerologic TestsTrachomaAntibodies, BacterialAntigens, BacterialBacterial Proteinspgp3 protein, ChlamydiaAntibodiesC. trachomatisNepalPopulation surveillanceTrachoma

Identifiers

PMID28214223
PMCPMC11025397

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.